Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A300-461A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse DDX6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody A300-461A (lot A300-461A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DDX6 by western blot. Samples: Whole cell lysate (2 µg) from HEK293T, HeLa, MCF-7, Jurkat, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody (Product # A300-461A; lot A300-461A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse DDX6 by western blot. Samples: Whole cell lysate (2 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody (Product # A300-461A; lot A300-461A-4) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX6 antibody A300-461A (lot A300-461A-3) used for IP at 6 µg per reaction. DDX6 was also immunoprecipitated by a previous lot of this antibody (A300-461A-2) and rabbit anti-DDX6 antibody A300-460A. For blotting immunoprecipitated DDX6, A300-461A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse DDX6 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX6 antibody A300-461A (lot A300-461A-3) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DDX6 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 (Product # A300-461A; lot 4) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DDX6 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- DDX6 Cat. No. A300-461A Lot 2 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human DDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 2% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX6 antibody (Product # A300-461A; lot A300-461A-4) used for IP at 6 µg per reaction. DDX6 was also immunoprecipitated by a previous lot of this antibody (lot A300-461A-3) and rabbit anti-DDX6 antibody (Product # A300-460A). For blotting immunoprecipitated DDX6 (Product # A300-461A-4) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
