Antibody data
- Antibody Data
- Antigen structure
- References [34]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- NB200-191 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-191, RRID:AB_10003156
- Product name
- Rabbit Polyclonal DDX6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182.
GC content shapes mRNA storage and decay in human cells.
Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration.
P-Body Purification Reveals the Condensation of Repressed mRNA Regulons.
YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1.
P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes.
Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia.
Multiple binding of repressed mRNAs by the P-body protein Rck/p54.
Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins.
Roles of ataxin-2 in pathological cascades mediated by TAR DNA-binding protein 43 (TDP-43) and Fused in Sarcoma (FUS).
The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography.
Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules.
RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.
The human Pat1b protein: a novel mRNA deadenylation factor identified by a new immunoprecipitation technique.
MicroRNA 130 family regulates the hypoxia response signal through the P-body protein DDX6.
Distinct functions of maternal and somatic Pat1 protein paralogs.
DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation.
Live-cell coimaging of the genomic RNAs and Gag proteins of two lentiviruses.
Defects in 18 S or 28 S rRNA processing activate the p53 pathway.
A role for huntington disease protein in dendritic RNA granules.
Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly.
Unravelling the ultrastructure of stress granules and associated P-bodies in human cells.
The virion-packaged endoribonuclease of herpes simplex virus 1 cleaves mRNA in polyribosomes.
Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies.
Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets.
Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells.
Human immunodeficiency virus type 1 Vif functionally interacts with diverse APOBEC3 cytidine deaminases and moves with them between cytoplasmic sites of mRNA metabolism.
Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation.
Dendrites of mammalian neurons contain specialized P-body-like structures that respond to neuronal activation.
3' Terminal oligo U-tract-mediated stimulation of decapping.
Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules.
Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.
Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54.
Sharma NR, Majerciak V, Kruhlak MJ, Yu L, Kang JG, Yang A, Gu S, Fritzler MJ, Zheng ZM
Nucleic acids research 2019 Sep 26;47(17):9368-9385
Nucleic acids research 2019 Sep 26;47(17):9368-9385
GC content shapes mRNA storage and decay in human cells.
Courel M, Clément Y, Bossevain C, Foretek D, Vidal Cruchez O, Yi Z, Bénard M, Benassy MN, Kress M, Vindry C, Ernoult-Lange M, Antoniewski C, Morillon A, Brest P, Hubstenberger A, Roest Crollius H, Standart N, Weil D
eLife 2019 Dec 19;8
eLife 2019 Dec 19;8
Staufen1 links RNA stress granules and autophagy in a model of neurodegeneration.
Paul S, Dansithong W, Figueroa KP, Scoles DR, Pulst SM
Nature communications 2018 Sep 7;9(1):3648
Nature communications 2018 Sep 7;9(1):3648
P-Body Purification Reveals the Condensation of Repressed mRNA Regulons.
Hubstenberger A, Courel M, Bénard M, Souquere S, Ernoult-Lange M, Chouaib R, Yi Z, Morlot JB, Munier A, Fradet M, Daunesse M, Bertrand E, Pierron G, Mozziconacci J, Kress M, Weil D
Molecular cell 2017 Oct 5;68(1):144-157.e5
Molecular cell 2017 Oct 5;68(1):144-157.e5
YB-1 regulates stress granule formation and tumor progression by translationally activating G3BP1.
Somasekharan SP, El-Naggar A, Leprivier G, Cheng H, Hajee S, Grunewald TG, Zhang F, Ng T, Delattre O, Evdokimova V, Wang Y, Gleave M, Sorensen PH
The Journal of cell biology 2015 Mar 30;208(7):913-29
The Journal of cell biology 2015 Mar 30;208(7):913-29
P-body assembly requires DDX6 repression complexes rather than decay or Ataxin2/2L complexes.
Ayache J, Bénard M, Ernoult-Lange M, Minshall N, Standart N, Kress M, Weil D
Molecular biology of the cell 2015 Jul 15;26(14):2579-95
Molecular biology of the cell 2015 Jul 15;26(14):2579-95
Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia.
de Vries S, Naarmann-de Vries IS, Urlaub H, Lue H, Bernhagen J, Ostareck DH, Ostareck-Lederer A
The Journal of biological chemistry 2013 Feb 22;288(8):5815-27
The Journal of biological chemistry 2013 Feb 22;288(8):5815-27
Multiple binding of repressed mRNAs by the P-body protein Rck/p54.
Ernoult-Lange M, Baconnais S, Harper M, Minshall N, Souquere S, Boudier T, Bénard M, Andrey P, Pierron G, Kress M, Standart N, le Cam E, Weil D
RNA (New York, N.Y.) 2012 Sep;18(9):1702-15
RNA (New York, N.Y.) 2012 Sep;18(9):1702-15
Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins.
Novotny I, Podolská K, Blazíková M, Valásek LS, Svoboda P, Stanek D
Molecular biology of the cell 2012 Oct;23(19):3776-85
Molecular biology of the cell 2012 Oct;23(19):3776-85
Roles of ataxin-2 in pathological cascades mediated by TAR DNA-binding protein 43 (TDP-43) and Fused in Sarcoma (FUS).
Nihei Y, Ito D, Suzuki N
The Journal of biological chemistry 2012 Nov 30;287(49):41310-23
The Journal of biological chemistry 2012 Nov 30;287(49):41310-23
The dual organization of P-bodies revealed by immunoelectron microscopy and electron tomography.
Cougot N, Cavalier A, Thomas D, Gillet R
Journal of molecular biology 2012 Jun 29;420(1-2):17-28
Journal of molecular biology 2012 Jun 29;420(1-2):17-28
Muscle satellite cells are primed for myogenesis but maintain quiescence with sequestration of Myf5 mRNA targeted by microRNA-31 in mRNP granules.
Crist CG, Montarras D, Buckingham M
Cell stem cell 2012 Jul 6;11(1):118-26
Cell stem cell 2012 Jul 6;11(1):118-26
RNA-related nuclear functions of human Pat1b, the P-body mRNA decay factor.
Marnef A, Weil D, Standart N
Molecular biology of the cell 2012 Jan;23(1):213-24
Molecular biology of the cell 2012 Jan;23(1):213-24
The human Pat1b protein: a novel mRNA deadenylation factor identified by a new immunoprecipitation technique.
Totaro A, Renzi F, La Fata G, Mattioli C, Raabe M, Urlaub H, Achsel T
Nucleic acids research 2011 Jan;39(2):635-47
Nucleic acids research 2011 Jan;39(2):635-47
MicroRNA 130 family regulates the hypoxia response signal through the P-body protein DDX6.
Saito K, Kondo E, Matsushita M
Nucleic acids research 2011 Aug;39(14):6086-99
Nucleic acids research 2011 Aug;39(14):6086-99
Distinct functions of maternal and somatic Pat1 protein paralogs.
Marnef A, Maldonado M, Bugaut A, Balasubramanian S, Kress M, Weil D, Standart N
RNA (New York, N.Y.) 2010 Nov;16(11):2094-107
RNA (New York, N.Y.) 2010 Nov;16(11):2094-107
DDX6 (Rck/p54) is required for efficient hepatitis C virus replication but not for internal ribosome entry site-directed translation.
Jangra RK, Yi M, Lemon SM
Journal of virology 2010 Jul;84(13):6810-24
Journal of virology 2010 Jul;84(13):6810-24
Live-cell coimaging of the genomic RNAs and Gag proteins of two lentiviruses.
Kemler I, Meehan A, Poeschla EM
Journal of virology 2010 Jul;84(13):6352-66
Journal of virology 2010 Jul;84(13):6352-66
Defects in 18 S or 28 S rRNA processing activate the p53 pathway.
Hölzel M, Orban M, Hochstatter J, Rohrmoser M, Harasim T, Malamoussi A, Kremmer E, Längst G, Eick D
The Journal of biological chemistry 2010 Feb 26;285(9):6364-70
The Journal of biological chemistry 2010 Feb 26;285(9):6364-70
A role for huntington disease protein in dendritic RNA granules.
Savas JN, Ma B, Deinhardt K, Culver BP, Restituito S, Wu L, Belasco JG, Chao MV, Tanese N
The Journal of biological chemistry 2010 Apr 23;285(17):13142-53
The Journal of biological chemistry 2010 Apr 23;285(17):13142-53
Angiogenin-induced tRNA-derived stress-induced RNAs promote stress-induced stress granule assembly.
Emara MM, Ivanov P, Hickman T, Dawra N, Tisdale S, Kedersha N, Hu GF, Anderson P
The Journal of biological chemistry 2010 Apr 2;285(14):10959-68
The Journal of biological chemistry 2010 Apr 2;285(14):10959-68
Unravelling the ultrastructure of stress granules and associated P-bodies in human cells.
Souquere S, Mollet S, Kress M, Dautry F, Pierron G, Weil D
Journal of cell science 2009 Oct 15;122(Pt 20):3619-26
Journal of cell science 2009 Oct 15;122(Pt 20):3619-26
The virion-packaged endoribonuclease of herpes simplex virus 1 cleaves mRNA in polyribosomes.
Taddeo B, Zhang W, Roizman B
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 21;106(29):12139-44
Proceedings of the National Academy of Sciences of the United States of America 2009 Jul 21;106(29):12139-44
Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies.
Ernoult-Lange M, Wilczynska A, Harper M, Aigueperse C, Dautry F, Kress M, Weil D
Molecular biology of the cell 2009 Jan;20(1):176-87
Molecular biology of the cell 2009 Jan;20(1):176-87
Identification of a cytoplasmic complex that adds a cap onto 5'-monophosphate RNA.
Otsuka Y, Kedersha NL, Schoenberg DR
Molecular and cellular biology 2009 Apr;29(8):2155-67
Molecular and cellular biology 2009 Apr;29(8):2155-67
Ribonomic analysis of human Pum1 reveals cis-trans conservation across species despite evolution of diverse mRNA target sets.
Morris AR, Mukherjee N, Keene JD
Molecular and cellular biology 2008 Jun;28(12):4093-103
Molecular and cellular biology 2008 Jun;28(12):4093-103
Deadenylation is prerequisite for P-body formation and mRNA decay in mammalian cells.
Zheng D, Ezzeddine N, Chen CY, Zhu W, He X, Shyu AB
The Journal of cell biology 2008 Jul 14;182(1):89-101
The Journal of cell biology 2008 Jul 14;182(1):89-101
Human immunodeficiency virus type 1 Vif functionally interacts with diverse APOBEC3 cytidine deaminases and moves with them between cytoplasmic sites of mRNA metabolism.
Marin M, Golem S, Rose KM, Kozak SL, Kabat D
Journal of virology 2008 Jan;82(2):987-98
Journal of virology 2008 Jan;82(2):987-98
Coordinated changes in mRNA turnover, translation, and RNA processing bodies in bronchial epithelial cells following inflammatory stimulation.
Zhai Y, Zhong Z, Chen CY, Xia Z, Song L, Blackburn MR, Shyu AB
Molecular and cellular biology 2008 Dec;28(24):7414-26
Molecular and cellular biology 2008 Dec;28(24):7414-26
Dendrites of mammalian neurons contain specialized P-body-like structures that respond to neuronal activation.
Cougot N, Bhattacharyya SN, Tapia-Arancibia L, Bordonné R, Filipowicz W, Bertrand E, Rage F
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Dec 17;28(51):13793-804
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Dec 17;28(51):13793-804
3' Terminal oligo U-tract-mediated stimulation of decapping.
Song MG, Kiledjian M
RNA (New York, N.Y.) 2007 Dec;13(12):2356-65
RNA (New York, N.Y.) 2007 Dec;13(12):2356-65
Ataxin-2 interacts with the DEAD/H-box RNA helicase DDX6 and interferes with P-bodies and stress granules.
Nonhoff U, Ralser M, Welzel F, Piccini I, Balzereit D, Yaspo ML, Lehrach H, Krobitsch S
Molecular biology of the cell 2007 Apr;18(4):1385-96
Molecular biology of the cell 2007 Apr;18(4):1385-96
Human retroviral host restriction factors APOBEC3G and APOBEC3F localize to mRNA processing bodies.
Wichroski MJ, Robb GB, Rana TM
PLoS pathogens 2006 May;2(5):e41
PLoS pathogens 2006 May;2(5):e41
Translation repression in human cells by microRNA-induced gene silencing requires RCK/p54.
Chu CY, Rana TM
PLoS biology 2006 Jul;4(7):e210
PLoS biology 2006 Jul;4(7):e210
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: DDX6 Antibody [NB200-191] - Detection of human DDX6 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX6 antibody NB200-191 used at 0.04 ug/ml for WB (A and B) and at 3 ug/mg lysate for IP. DDX6 was also immunoprecipitated using rabbit anti-DDX6 antibody NB200-192, but not with 'DDX6 Ab', which is to another epitope from DDX6. Normal rabbit IgG was used as a negative control. Detection: Chemiluminescence with an exposure time of 1 second (A & B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: DDX6 Antibody [NB200-191] - Sample: FFPE section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-191] - Section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-DDX6 used at a dilution of 1:200 (1ug/ml). Detection: DAB.