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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA024258 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA024258, RRID:AB_1847795
- Product name
- Anti-DNAJB6
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human DNAJB6, Gene description: DnaJ (Hsp40) homolog, subfamily B, member 6, Alternative Gene Names: LGMD1D, MRJ, Validated applications: IHC, WB, Uniprot ID: O75190, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Single-Cell RNA Sequencing of Human, Macaque, and Mouse Testes Uncovers Conserved and Divergent Features of Mammalian Spermatogenesis
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Shami A, Zheng X, Munyoki S, Ma Q, Manske G, Green C, Sukhwani M, Orwig K, Li J, Hammoud S
Developmental Cell 2020;54(4):529-547.e12
Developmental Cell 2020;54(4):529-547.e12
Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323
Nature Methods 2013;10(4):315-323
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and DNAJB6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409211).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and pancreas tissues using Anti-DNAJB6 antibody. Corresponding DNAJB6 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
