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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00010049-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010049-M02, RRID:AB_10628147
- Product name
- DNAJB6 monoclonal antibody (M02), clone 2D12-B9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant DNAJB6.
- Antigen sequence
MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKN
PENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGK
EGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFG
GRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSF
FSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSF
SSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVE
NGQERVEVEEDGQLKSLTINGKEQLLRLDNK- Isotype
- IgG
- Antibody clone number
- 2D12-B9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DNAJB6 expression in transfected 293T cell line by DNAJB6 monoclonal antibody (M02), clone 2D12-B9.Lane 1: DNAJB6 transfected lysate (Predicted MW: 36.1 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DNAJB6 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to DNAJB6 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNAJB6 transfected lysate using anti-DNAJB6 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DNAJB6 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
