PA5-55031

antibody from Invitrogen Antibodies

Targeting: DNAJB6 LGMD1D, MRJ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-55031

Provider

Invitrogen Antibodies

Product name

DNAJB6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FDSPFEFGFT FRNPDDVFRE FFGGRDPFSF DFFEDPFEDF FGNRRGPRGS RSRGTGSFFS AFSGFPSFGS GFSSFDTGFT SFGSLGHGGL TSFSSTS Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 94%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.05 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DNAJB6 in control (vector only transfected HEK293T lysate) and DNAJB6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DNAJB6 Polyclonal Antibody (Product # PA5-55031).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DNAJB6 in control (vector only transfected HEK293T lysate) and DNAJB6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a DNAJB6 Polyclonal Antibody (Product # PA5-55031).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of DNAJB6 in human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a DNAJB6 Polyclonal Antibody (Product # PA5-55031).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DNAJB6 in human testis and pancreas tissues using DNAJB6 Polyclonal Antibody (Product # PA5-55031). Corresponding DNAJB6 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DNAJB6 in human pancreas using DNAJB6 Polyclonal Antibody (Product # PA5-55031) shows low expression as expected.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of DNAJB6 in human testis using DNAJB6 Polyclonal Antibody (Product # PA5-55031) shows high expression.