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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-121060 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNAJB6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: SW480, MCF7, 293T, Jurkat, HeLa, Mouse testis, Mouse brain, Rat brain. The target is usually found in the following locations: Cytoplasm, Nucleus, Z line, myofibril, perinuclear region, sarcomere. Immunogen sequence: STSFGGSGMG NFKSISTSTK MVNGRKITTK RIVENGQERV EVEEDGQLKS LTINGVADDD ALAEERMRRG QNALPAQPAG LRPPKPPRPA SLLRHAPHCL SEEEGEQDRP RAPGPWDPLA SAAGLKEGGK RKKQKQREES KKKKSTKGNH
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNAJB6 in extracts of various cell lines. Samples were incubated with DNAJB6 Polyclonal antibody (Product # PA5-121060) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DNAJB6 in U2OS cells. Samples were incubated with DNAJB6 Polyclonal antibody (Product # PA5-121060). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNAJB6 in paraffin-embedded human kidney cancer. Samples were incubated with DNAJB6 Polyclonal antibody (Product # PA5-121060) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNAJB6 in paraffin-embedded mouse ileum. Samples were incubated with DNAJB6 Polyclonal antibody (Product # PA5-121060) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNAJB6 in paraffin-embedded human kidney cancer. Samples were incubated with DNAJB6 Polyclonal antibody (Product # PA5-121060) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNAJB6 in paraffin-embedded mouse ileum. Samples were incubated with DNAJB6 Polyclonal antibody (Product # PA5-121060) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
