PA5-27577

antibody from Invitrogen Antibodies

Targeting: DNAJB6 LGMD1D, MRJ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27577

Provider

Invitrogen Antibodies

Product name

DNAJB6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Molt-4, MDA-MB-231.

Concentration

0.58 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DNAJB6 using 30 µg of U87-MG lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a DNAJB6 polyclonal antibody (Product # PA5-27577) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DNAJB6 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNAJB6 Polyclonal Antibody (Product # PA5-27577) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DNAJB6 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNAJB6 Polyclonal Antibody (Product # PA5-27577) at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DNAJB6 Polyclonal Antibody [N3C3] detects DNAJB6 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNAJB6 protein stained by DNAJB6 Polyclonal Antibody [N3C3] (Product # PA5-27577) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.