Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- PA5-27577 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNAJB6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Molt-4, MDA-MB-231. Predicted reactivity: Rhesus Monkey (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.58 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DNAJB6 using 30 µg of U87-MG lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a DNAJB6 polyclonal antibody (Product # PA5-27577) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNAJB6 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNAJB6 Polyclonal Antibody (Product # PA5-27577) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNAJB6 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DNAJB6 Polyclonal Antibody (Product # PA5-27577) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DNAJB6 Polyclonal Antibody [N3C3] detects DNAJB6 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNAJB6 protein stained by DNAJB6 Polyclonal Antibody [N3C3] (Product # PA5-27577) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.