PA5-23407

antibody from Invitrogen Antibodies

Targeting: MNX1 HB9, HLXB9, HOXHB9, SCRA1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-23407

Provider

Invitrogen Antibodies

Product name

HB9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Cdo Is Required for Efficient Motor Neuron Generation of Embryonic Stem Cells.

Kim H, Lee SY, Jeong HJ, Kang JS, Cho H, Leem YE
International journal of stem cells 2020 Nov 30;13(3):342-352

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Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients.

Kim KY, Kim YR, Choi KW, Lee M, Lee S, Im W, Shin JY, Kim JY, Hong YH, Kim M, Kim JI, Sung JJ
Translational neurodegeneration 2020 Jul 1;9(1):23

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of HB9 in Neuro2a cells fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. Samples were incubated in HB9 polyclonal antibody (Product # PA5-23407) using a dilution of 1 µg/mL for 60 minutes at room temperature followed by anti-rabbit DyLight 488 (Green) with a dilution of 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 Downregulated miR-18b (miR-18b-5p) by SOD1 mutations is associated with apoptotic cell death in motor neuron from hiPSC-derived NSCs fALS SOD1 (G17S). a Nanog (green) and Oct4 (red) iPSC marker are expressed in hiPSCs (normal and fALS SOD1 (G17S). Scale bar, 20 mum. b Neural stem cells (SOX2 and Nestin) was generated from hiPSCs (normal and fALS SOD1 (G17S)). Scale bar, 40 mum. c Differentiated motor neurons from hNSCs were double-stained with HB9 and ChAT antibodies. Scale bar, 20 mum. d Hif1alpha and Mef2c transcripts were upregulated in differentiated motor neurons SOD1 (G17S). e The mRNAs levels of Mctp1 and Rarb was downregulated in differentiated motor neurons SOD1 (G17S). f Intracellular Ca 2+ levels (Normal (0.149) versus SOD1 (G17S) (0.215) in fluorescence intensities from baseline 490/525 ratio) were enhanced in differentiated motor neurons SOD1 (G17S). Total neurite length was reduced in differentiated motor neurons SOD1 (G17S). g miR-18b (miR-18b-5p) expression was deeply low and mir-206 was highly expression in differentiated motor neurons SOD1 (G17S). Significantly different at *, p < 0.05; **, p < 0.005. The experiments were replicated 5 times