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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA043903 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA043903, RRID:AB_2678724
- Product name
- Anti-ACAD8
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ACAD8, Gene description: acyl-CoA dehydrogenase family, member 8, Validated applications: WB, IHC, Uniprot ID: Q9UKU7, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Acyl‐CoA dehydrogenase substrate promiscuity: Challenges and opportunities for development of substrate reduction therapy in disorders of valine and isoleucine metabolism
Houten S, Dodatko T, Dwyer W, Violante S, Chen H, Stauffer B, DeVita R, Vaz F, Cross J, Yu C, Leandro J
Journal of Inherited Metabolic Disease 2023;46(5):931-942
Journal of Inherited Metabolic Disease 2023;46(5):931-942
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and ACAD8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415328).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human kidney and lymph node tissues using HPA043903 antibody. Corresponding ACAD8 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
