F54574

antibody from NSJ Bioreagents

Targeting: PARS2 DKFZp727A071

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54574

Provider

NSJ Bioreagents

Product name

Prolyl-tRNA synthetase Antibody / ProRS

Antibody type

Polyclonal

Description

This highly specific Prolyl-tRNA synthetase antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Prolyl-tRNA synthetase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Prolyl-tRNA synthetase antibody. Predicted molecular weight ~53 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse bladder tissue lysate with Prolyl-tRNA synthetase antibody. Predicted molecular weight ~53 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human kidney tissue with Prolyl-tRNA synthetase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HEK293 cells with Prolyl-tRNA synthetase antibody; Blue=isotype control, Green= Prolyl-tRNA synthetase antibody.