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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab131081 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Thymidine Kinase 1/TK1 Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human Thymidine Kinase 1/TK1 Antibody, Monoclonal (C3), could be used for WB, ICC, IF and so on.ApplicationWB: 0.01-6 µg/mLIHC: 5-50 µg/mL
- Reactivity
- Human, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 2-234- Antibody clone number
- C3
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) - Western Blot All lanes: Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution Predicted band size: 25 kDa Observed band size: 26 kDa Exposure time: 60.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) - IF/ICC FITC staining on IF Samples: Human MCF7 cells Primary Ab: 20 µg/mL Thymidine Kinase 1/TK1 Mouse mAb (Ab131081) Second Ab: 1 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
