PA5-29686

antibody from Invitrogen Antibodies

Targeting: TK1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29686

Provider

Invitrogen Antibodies

Product name

TK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, A431 cytoplasm extract.

Concentration

1.01 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Thymidine Kinase 1 using 30 µg of A431 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Thymidine Kinase 1 polyclonal antibody (Product # PA5-29686) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TK1 was performed by separating 30 µg of A431 whole cell and cytoplasm extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a TK1 Polyclonal Antibody (Product # PA5-29686) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TK1 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a TK1 Polyclonal Antibody (Product # PA5-29686) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Thymidine Kinase 1 (Product # PA5-29686) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

TK1 Polyclonal Antibody detects Thymidine Kinase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat ovary. Thymidine Kinase 1 stained by TK1 Polyclonal Antibody (Product # PA5-29686) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse muscle, using Thymidine Kinase 1 (Product # PA5-29686) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.