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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-27944 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DHCR24 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, mouse brain. Predicted reactivity: Mouse (95%), Rat (95%), Zebrafish (82%), Xenopus laevis (81%), Pig (95%), Chicken (80%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.29 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Seladin 1 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Seladin 1 polyclonal antibody (Product # PA5-27944) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Seladin 1 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Seladin 1 polyclonal antibody (Product # PA5-27944) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DHCR24 Polyclonal Antibody (Product # PA5-27944). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DHCR24 Polyclonal Antibody (Product # PA5-27944) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Seladin 1 in paraformaldehyde-fixed HeLa cells using a Seladin 1 polyclonal antibody (Product # PA5-27944) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Seladin 1 (Product # PA5-27944) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DHCR24 Polyclonal Antibody detects Seladin 1 protein at cytoplasm on rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. DHCR24 Polyclonal Antibody (Product # PA5-27944) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
