Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- ABIN359035 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Aurora Kinase C (AURKC) (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide selected from the N-terminal region of Human Aurora-C. Genename: AURKC
- Description
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- N-Term
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Cell cycle-dependent expression and centrosome localization of a third human aurora/Ipl1-related protein kinase, AIK3.
Protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (AIE1, AIE2) related to yeast and fly chromosome segregation regulators.
Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter.
Kimura M, Matsuda Y, Yoshioka T, Okano Y
The Journal of biological chemistry 1999 Mar 12;274(11):7334-40
The Journal of biological chemistry 1999 Mar 12;274(11):7334-40
Protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (AIE1, AIE2) related to yeast and fly chromosome segregation regulators.
Tseng TC, Chen SH, Hsu YP, Tang TK
DNA and cell biology 1998 Oct;17(10):823-33
DNA and cell biology 1998 Oct;17(10):823-33
Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter.
Bernard M, Sanseau P, Henry C, Couturier A, Prigent C
Genomics 1998 Nov 1;53(3):406-9
Genomics 1998 Nov 1;53(3):406-9
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot: AP13519PU-N Aurora C antibody staining of lysates of 293 cells expressing Flag tag (Lane 1), Flag-tagged Aurora A (Lane 2), Flag-tagged Aurora B (Lane 3) or Flag-tagged Aurora C (Lane 4).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence: AP13519PU-N Aurora C antibody staining of HeLa cells, expressing GFP-Aurora C, at different cellular mitotic stages as primary antibody (A), GFP Fluorescence (B), DAPI nuclear staining (C), and AP13519PU-N merged to DAPI staining (D). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).