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Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Other assay [1]
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- Product number
- PA1-12680 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPAP2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for this product is transfected HEK-293 cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Chromatin-modifying agents convert fibroblasts to OCT4+ and VEGFR-2+ capillary tube-forming cells.
Wary A, Wary N, Baruah J, Mastej V, Wary KK
PloS one 2017;12(5):e0176496
PloS one 2017;12(5):e0176496
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 2 Chromatin modification mediate expression of mesodermal and endothelial cells marker proteins. Total protein extracts prepared from: a) d2 control cells; b) d3 cells treated once with Aza + TSA; c) d4 cells treated twice with Aza + TSA; d) d5, treated with a third dose of Aza + TSA and TDG were resolved by denaturing SDS-PAGE. Proteins were transferred onto a nitrocellulose membrane and analyzed by Western blotting (WB) with antibodies to: A ) beta-catenin, B ) OCT4, and C ) VEGFR-2/FLK1; D ) Brachyury; E ) N-cadherin; F ) TIE-2; G ) CD31; H ) LPP3; and ( I ) beta-tubulin. beta-tubulin antibody was used to judge equal loading of proteins across the lanes. ( J ) Representative image of ponceau-S stained nitrocellulose membrane. Molecular weights are given in kiloDaltons (kDa). The numbers at the lowermost rows of each image indicate signal intensities that were quantified by NIH ImageJ. Experiments were carried out three times.
