Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A304-603A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H1x Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human Histone H1x by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Histone H1x antibody A304-603A (lot A304-603A-1) used for IP at 6 µg per reaction. Histone H1x was also immunoprecipitated by rabbit anti-Histone H1x antibody A304-604A. For blotting immunoprecipitated Histone H1x, A304-603A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.