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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-78184 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-MST1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: 293T, A431, HeLa, HepG2 Predicted Reactivity: Mouse (95%), Rat (92%), Chicken (88%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (94%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.23 mg/ml
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MST1 in whole cell lysate using 30 µg of protein. Samples were separated with 10% SDS-PAGE and incubated with MST1 polyclonal antibody (Product # PA5-78184) using a dilution of 1:1000 followed by HRP-conjugated anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MST1 in paraffin-embedded human lung carcinoma. Sample was incubated with MST1 polyclonal antibody (Product # PA5-78184) using a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MST1 in PC-3 cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with MST1 polyclonal antibody (Product # PA5-78184) using a dilution of 1:500, followed by Hoechst.
