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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-120917 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MERTK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: HepG2, Mouse spleen. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein. Immunogen sequence: REEAKPYPLF PGPFPGSLQT DHTPLLSLPH ASGYQPALMF SPTQPGRPHT GNVAIPQVTS VESKPLPPLA FKHTVGHIIL SEHKGVKFNC SISVPNIYQD TTISWWKDGK ELLGAHHAIT QFYPDDEVTA IIASFSITSV QRSDNGSYIC KMKINNEEIV SDPIYIEVQG LPHFTKQPES MNVTRNTAFN LTCQAVGPPE PVNIFWVQNS SRVNEQPEKS PSVLTVPGLT EMAVFSCEAH NDKGLTVSKG VQINIKAIPS PPTEVSIRNS TAHSILISWV PGFDGYSPFR NCSIQVKEAD PLSNGSVMIF NTSALPHLYQ IKQLQALANY SIGVSCMNEI GWSAVSPWIL ASTTEGAPSV APLNVTVFLN ESSDNVDIRW MKPPTKQQDG ELVGYRISHV WQSAGISKEL LEEVGQNGSR ARISVQVHNA TCTVRIAAVT RGGVGPFSDP VKIFIPAHGW VDYAPSSTPA PGNA
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.75 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MERTK in extracts of Mouse spleen. Samples were incubated with MERTK Polyclonal antibody (Product # PA5-120917) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MERTK in extracts of HepG2 cells. Samples were incubated with MERTK Polyclonal antibody (Product # PA5-120917) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of MERTK in U2OS cells. Samples were incubated with MERTK Polyclonal antibody (Product # PA5-120917) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of MERTK in paraffin-embedded rat spleen. Samples were incubated with MERTK Polyclonal antibody (Product # PA5-120917) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
