ATR-033-200UL

antibody from Invitrogen Antibodies

Targeting: MERTK c-Eyk, mer, RP38, Tyro12

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

ATR-033-200UL

Provider

Invitrogen Antibodies

Product name

MERTK (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-MERTK (extracellular) Antibody (#ATR-033), (1:400). 3, 4. Anti-MERTK (extracellular) Antibody , preincubated with MERTK (extracellular) Blocking Peptide (#BLP-TR033).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis human HepG2 hepatocellular carcinoma cell line lysate (lanes 1 and 4), mouse BV-2 microglia cell line lysate (lanes 2 and 5) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 3 and 6): - 1-3. Anti-MERTK (extracellular) Antibody (#ATR-033), (1:400). 4-6. Anti-MERTK (extracellular) Antibody , preincubated with MERTK (extracellular) Blocking Peptide (#BLP-TR033).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1, 2. Anti-MERTK (extracellular) Antibody (#ATR-033), (1:400). 3, 4. Anti-MERTK (extracellular) Antibody , preincubated with MERTK (extracellular) Blocking Peptide (#BLP-TR033).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of MERTK in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MERTK (extracellular) Antibody (#ATR-033), (1:300), followed by goat anti rabbit conjugated with Alexa 488 (green). A. MERTK immunoreactivity appears in in blood vessel profiles (vertical arrows) in the molecular (M), purkinje (P) and granule (G) layers and in basket profiles (horizontal arrows) around Purkinje soma. B. Pre-incubation of the Antibody with MERTK (extracellular) Blocking Peptide (#BLP-TR033), suppresses staining. Cell nuclei are stained with DAPI (blue). G= Granule layer, P = Purkinje layer, M = Molecular layer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of MERTK in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-MERTK (extracellular) Antibody (#ATR-033), (1:300), followed by goat Anti-rabbit-AlexaFluor-488 (green). A. MERTK staining (green) in the mouse hippocampal dentate gyrus region is detected in blood vessel profiles (arrows) in the hilus (H) and outer molecular layer (OML). B. Pre-incubation of the Antibody with MERTK (extracellular) Blocking Peptide (#BLP-TR033), suppresses staining. Cell nuclei are stained with DAPI (blue). G = Granule layer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Multiplex staining of MERTK and Aquaporin 4 in rat parietal cortex. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-MERTK (extracellular) Antibody (#ATR-033), (1:300), followed by goat Anti-rabbit-AlexaFluor-488 and Guinea Pig Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014-GP), (1:300), followed by goat Anti-guinea pig-AlexaFluor-594. A. MERTK immunoreactivity (greens) appears around blood vessels (arrows). B. Aquaporin 4 immunoreactivity (red) appears around blood vessels (arrows). C. Merge of the two images reveals several vessels that express both MERTK and AQP4 (arrows point at examples).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of MERTK in live intact mouse BV-2 microglia cell line: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-MERTK (extracellular) Antibody (#ATR-033), (5 µg) + goat- Anti-rabbit-FITC.