PA5-15028

antibody from Invitrogen Antibodies

Targeting: MERTK c-Eyk, mer, RP38, Tyro12

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-15028

Provider

Invitrogen Antibodies

Product name

MERTK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

400 µL

Concentration

2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a MERTK polyclonal antibody (Product # PA5-15028) in 293 cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MERTK Polyclonal Antibody (Product # PA5-15028) and a 110kDa band corresponding to Tyrosine-protein kinase Mer was observed across cell lines and tissues tested . Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), HEL 92.1.7 (Lane 2), A549 (Lane 3), A549 treated with 80nM Doxorubicin for 48h (Lane 4), THP-1 (Lane 5), THP-1 treated with 100nM Actinomycin D for 8h (Lane 6), THP-1 (Lane 7), THP-1 treated with 2µM Thapsigargin D for 8h (Lane 8), HepG2 (Lane 9), Mouse Spleen (Lane 10), Rat Heart (Lane 11) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).MERTK expression increased in A-549 cells upon treatment with Doxorubicin, decreased in THP-1 cells upon treatment with Actinomycin D and Thapsigargin as seen. A non specific band was observed around 20kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of U251 cells using a MERTK polyclonal antibody (Product # PA5-15028). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a MERTK polyclonal antibody (Product # PA5-15028) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of U251 cells using a MERTK polyclonal antibody (Product # PA5-15028). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a MERTK polyclonal antibody (Product # PA5-15028) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 µg/mL, 10 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymphoma tissue using a MERTK polyclonal antibody (Product # PA5-15028), followed by HRP-conjugated secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Jurkat cells using a MERTK polyclonal antibody (Product # PA5-15028) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody