Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
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- Product number
- AF891 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Mer Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Mer in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Mer and less than 1% cross-reactivity with recombinant human (rh) Axl and rhDtk is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q12866
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Protein S and Gas6 induce efferocytosis of HIV-1-infected cells.
Human Sertoli cells support high levels of Zika virus replication and persistence.
Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation.
AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses.
Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma.
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation.
IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
Identification of Axl as a downstream effector of TGF-β1 during Langerhans cell differentiation and epidermal homeostasis.
Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages.
Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for Mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for Mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
Chua BA, Ngo JA, Situ K, Ramirez CM, Nakano H, Morizono K
Virology 2018 Feb;515:176-190
Virology 2018 Feb;515:176-190
Human Sertoli cells support high levels of Zika virus replication and persistence.
Kumar A, Jovel J, Lopez-Orozco J, Limonta D, Airo AM, Hou S, Stryapunina I, Fibke C, Moore RB, Hobman TC
Scientific reports 2018 Apr 3;8(1):5477
Scientific reports 2018 Apr 3;8(1):5477
Viral Infection Sensitizes Human Fetal Membranes to Bacterial Lipopolysaccharide by MERTK Inhibition and Inflammasome Activation.
Cross SN, Potter JA, Aldo P, Kwon JY, Pitruzzello M, Tong M, Guller S, Rothlin CV, Mor G, Abrahams VM
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 15;199(8):2885-2895
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 15;199(8):2885-2895
AXL-dependent infection of human fetal endothelial cells distinguishes Zika virus from other pathogenic flaviviruses.
Richard AS, Shim BS, Kwon YC, Zhang R, Otsuka Y, Schmitt K, Berri F, Diamond MS, Choe H
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):2024-2029
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):2024-2029
Expression and role of TYRO3 and AXL as potential therapeutical targets in leiomyosarcoma.
Dantas-Barbosa C, Lesluyes T, Loarer FL, Chibon F, Treilleux I, Coindre JM, Meeus P, Brahmi M, Bally O, Ray-Coquard I, Sunyach MP, Cesne AL, Mir O, Bonvalot S, Toulmonde M, Italiano A, Saintigny P, Jean-Denis M, Ducimetiere F, Ranchere D, El Sayadi H, Alberti L, Blay JY
British journal of cancer 2017 Dec 5;117(12):1787-1797
British journal of cancer 2017 Dec 5;117(12):1787-1797
MerTK cleavage limits proresolving mediator biosynthesis and exacerbates tissue inflammation.
Cai B, Thorp EB, Doran AC, Subramanian M, Sansbury BE, Lin CS, Spite M, Fredman G, Tabas I
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):6526-31
Proceedings of the National Academy of Sciences of the United States of America 2016 Jun 7;113(23):6526-31
IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.
Zizzo G, Cohen PL
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5237-46
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5237-46
Identification of Axl as a downstream effector of TGF-β1 during Langerhans cell differentiation and epidermal homeostasis.
Bauer T, Zagórska A, Jurkin J, Yasmin N, Köffel R, Richter S, Gesslbauer B, Lemke G, Strobl H
The Journal of experimental medicine 2012 Oct 22;209(11):2033-47
The Journal of experimental medicine 2012 Oct 22;209(11):2033-47
Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.
Zizzo G, Hilliard BA, Monestier M, Cohen PL
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 1;189(7):3508-20
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 1;189(7):3508-20
Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages.
Eken C, Martin PJ, Sadallah S, Treves S, Schaller M, Schifferli JA
The Journal of biological chemistry 2010 Dec 17;285(51):39914-21
The Journal of biological chemistry 2010 Dec 17;285(51):39914-21
Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for Mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
Uehara H, Shacter E
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2522-30
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2522-30
Auto-oxidation and oligomerization of protein S on the apoptotic cell surface is required for Mer tyrosine kinase-mediated phagocytosis of apoptotic cells.
Uehara H, Shacter E
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2522-30
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 15;180(4):2522-30
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Mer by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line and PANC-1 human pancreatic carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 170-230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human Mer Specificity by Using Knockout Cell Line. Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and Mer knockout HepG2 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Mer at approximately 175 kDa (as indicated) in the parental HepG2 cell line, but is not detectable in knockout HepG2 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.