A302-266A-T

antibody from Invitrogen Antibodies

Targeting: SASH1 dJ323M4.1, KIAA0790, SH3D6A

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-266A-T

Provider

Invitrogen Antibodies

Product name

SASH1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SASH1 by immunohistochemistry. Sample: FFPE sections of human breast carcinoma (left) and lung carcinoma (right). Antibody: Affinity purified rabbit anti- SASH1 (Cat. No. A302-266A Lot1) used at a dilution of 1:1,000 (1µg/ml) and 1:400 (2.5 µg/ml). Detection: DAB and Red-fluorescent goat anti-rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SASH1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SASH1 antibody A302-266A used for IP at 3 µg/mg lysate. SASH1 was also immunoprecipitated by rabbit anti-SASH1 antibody A302-265A, which recognizes an upstream epitope. For blotting immunoprecipitated SASH1, A320-265A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.