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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-47265 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Carbonic Anhydrase III Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) CA1, rhCA2, rhCA4, rhCA9, rhCA10, rhCA13 and rhCA14 is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of Carbonic Anhydrase III in human, mouse, and rat skeletal muscle tissue and heart tissue. Samples were incubated in Carbonic Anhydrase III polyclonal antibody (Product # PA5-47265) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Carbonic Anhydrase III/CA3 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions.
