PA5-47030

antibody from Invitrogen Antibodies

Targeting: TACSTD2 EGP-1, GA733-1, M1S1, TROP2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47030

Provider

Invitrogen Antibodies

Product name

TROP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) MCAM, rhNCAM-L1, and rhBCAM is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

SARS-CoV-2 can infect and propagate in human placenta explants.

Fahmi A, Brügger M, Démoulins T, Zumkehr B, Oliveira Esteves BI, Bracher L, Wotzkow C, Blank F, Thiel V, Baud D, Alves MP
Cell reports. Medicine 2021 Dec 21;2(12):100456

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of TROP2 was performed using 70% confluent log phase MCF-7 cells treated with 5-aza-2-deoxycytidine (2.5 µM for 48 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TROP2 Polyclonal Antibody (Product # PA5-47030) at 2 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # A27012) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing increased TROP2 expression and localization to plasma membrane upon treatment with 5-aza-2-deoxycytidine. Panel e shows untreated cells with lower expression of TROP2. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PC-3 human prostate cancer cell line was stained with Goat Anti-human TROP-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47030, filled histogram) or control antibodyopen histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of TROP2 in PC‚3 human prostate cancer cell line. Samples were incubated in TROP2 polyclonal antibody (Product # PA5-47030) or control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.