Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- PA5-47074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TROP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 30% cross-reactivity with recombinant human TROP-2 is observed.
- Concentration
- 0.2 mg/mL
Submitted references Serotonin transporter protects the placental cells against apoptosis in caspase 3-independent pathway.
Hadden C, Fahmi T, Cooper A, Savenka AV, Lupashin VV, Roberts DJ, Maroteaux L, Hauguel-de Mouzon S, Kilic F
Journal of cellular physiology 2017 Dec;232(12):3520-3529
Journal of cellular physiology 2017 Dec;232(12):3520-3529
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of TROP2 was achieved by transfecting MCF 10A cells with TROP2 specific siRNAs (Silencer® select Product # s8364, s8366). Western blot analysis (Fig. a) was performed using whole cell extracts from the TROP2 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Anti-TROP2 Polyclonal Antibody (Product # PA5-47074, 0.1 µg/ml) and Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Densitometric analysis of this Western Blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TROP2.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-TROP2 Polyclonal Antibody (Product # PA5-47074) and ~35kDa corresponding to total protein and a slightly higher glycosylated form at ~50kDa corresponding to TROP2 was observed in all cell lines and tissues tested except in Mouse colon which is a low expressing tissue. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MCF-7 treated with 5-Aza-2-deoxycytidine (2.5uM for 48 hrs) (Lane 2), MCF10A (Lane 3), MDA-MB-231 (Lane 4), NIH/3T3 (Lane 5), Rat kidney (Lane 6), Mouse skin (Lane 7), Mouse kidney (Lane 8), Mouse stomach (Lane 9), and Mouse colon (Lane 10) were electrophoresed using NuPAGE® 4-12% Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (0.1µg/ml) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TROP2 in immersion fixed XB2 mouse teratoma keratinocyte cell line. Samples were incubated in TROP2 polyclonal antibody (Product # PA5-47074) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TROP2 in immersion fixed paraffin-embedded sections of mouse skin. Samples were incubated with TROP2 polyclonal antibody (Product # PA5-47074) using a dilution of 3 µg/mL for 1 hour at room temperature followed by Anti-Goat IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in keratinocytes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of XB2 mouse teratoma keratinocyte cell line was stained with mouse TROP-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47074, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TROP2 in mIMCD-3 mouse epithelial cell line. Samples were incubated in TROP2 polyclonal antibody (Product # PA5-47074) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.