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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [2]
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- Product number
- PA5-47074 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TROP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, approximately 30% cross-reactivity with recombinant human TROP-2 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Serotonin transporter protects the placental cells against apoptosis in caspase 3-independent pathway.
Hadden C, Fahmi T, Cooper A, Savenka AV, Lupashin VV, Roberts DJ, Maroteaux L, Hauguel-de Mouzon S, Kilic F
Journal of cellular physiology 2017 Dec;232(12):3520-3529
Journal of cellular physiology 2017 Dec;232(12):3520-3529
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of TROP2 in immersion fixed XB2 mouse teratoma keratinocyte cell line. Samples were incubated in TROP2 polyclonal antibody (Product # PA5-47074) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of XB2 mouse teratoma keratinocyte cell line was stained with mouse TROP-2 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47074, filled histogram) or isotype control antibodyopen histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of TROP2 in mIMCD-3 mouse epithelial cell line. Samples were incubated in TROP2 polyclonal antibody (Product # PA5-47074) or isotype control antibody followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody.
