PA5-57331

antibody from Invitrogen Antibodies

Targeting: TRH

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-57331 - Provider product page
Provider: Invitrogen Antibodies
Product name: TRH Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant full-length protein
Description: Immunogen sequence: HKRQHPGRRE DEASWSVDVT QHKRQHPGRR SPWLAYAVPK RQHPGRRLAD PKAQRSWEE Highest antigen sequence identity to the following orthologs: Mouse - 51%, Rat - 50%.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.50 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TRH protein structure - PA5-57331 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 2 Figure Thyrotrophin-releasing hormone (TRH) neurones in the paraventricular nucleus of the hypothalamus of rats are directly responsive to leptin. A-F, Epifluorescence photomicrographs showing the co-localisation between phosphorylated signal transducer and activator of transcription 3 (pSTAT3) (green staining) and proTRH (red staining) in rats that received an i.c.v. injection of leptin (A-C) and in rats that did not receive leptin injection (D-F). The arrows indicate examples of double-labelled neurones. Abbreviations: 3V, third ventricle; ARH, arcuate nucleus; PVH, paraventricular nucleus of the hypothalamus; VMH, ventromedial nucleus. Scale bar = 50 um in (A) and (D); 20 um in (B) and (E); 100 um in (C) and (F)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 3 Figure By contrast to rats, thyrotrophin-releasing hormone (TRH) neurones in the hypothalamic paraventricular nucleus of mice are mostly unresponsive to leptin. A-I, Epifluorescence photomicrographs showing the co-localisation between phosphorylated signal transducer and activator of transcription 3 (pSTAT3) (green staining) and proTRH (red staining) in mice that received an i.p. injection of leptin (A-C) and in mice that received an i.c.v. injection of leptin (D-F), as well as the co-localisation between leptin receptor (LepR) and proTRH in LepR-reporter mice (G-I). The arrows indicate examples of double-labelled neurones, whereas arrowheads indicate leptin-responsive neurones that do not express proTRH. Abbreviations: 3V, third ventricle; ARH, arcuate nucleus; PVH, paraventricular nucleus of the hypothalamus; VMH, ventromedial nucleus. Scale bar = 50 um in (A), (D) and (G); 20 um in (B), (E) and (H); 100 um in (C), (F) and (I)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 4 Figure Leptin induces phosphorylation of the cAMP response element binding protein (pCREB) in thyrotrophin-releasing hormone (TRH) neurones of rats, but not in mice. A-C, Co-localisation between pCREB (green staining) and proTRH (red staining) in the paraventricular nucleus of the hypothalamus (PVH) of rats (n = 3 per group). D-F, Co-localisation between pCREB and proTRH in the PVH of mice (n = 4-6 per group). The arrows indicate examples of double-labelled neurones. Abbreviation: 3V, third ventricle. Scale bar = 100 um. *** P = 0.0007