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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Product number
- PA5-70781 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL6A5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 93%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 86%; Rabbit: 79%; Rat: 86%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Inhibition of Col6a5 Improve Lipid Metabolism Disorder in Dihydrotestosterone-Induced Hyperandrogenic Mice.
Sun LF, Yang YL, Wang MY, Zhao HS, Xiao TX, Li MX, Wang BB, Huang C, Ren PG, Zhang JV
Frontiers in cell and developmental biology 2021;9:669189
Frontiers in cell and developmental biology 2021;9:669189
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Inhibition of Col6a5 expression alleviated the hypertrophy of ovaries and gonadal fat pads in DHT-treated mice. (A) Relative expression of Col6a5 mRNA in the ovaries and gonadal fat pads showing a significant increase in Col6a5 expression after DHT treatment; n = 6. The data are expressed as the mean +- SEM and were compared by Mann-Whitney U -test. (B) Immunohistochemistry of Col6a5 in the ovaries showing a significant positive signal in ovarian stromal cells; n = 3, top, scale bars = 200 mum; bottom, scale bars = 20 mum. (C) Immunohistochemistry of PLIN2 (a marker of lipid droplets) in the ovaries showing a significant positive signal in hypertrophic ovarian stromal cells (GC, granulosa cells, TC, thecal cells); n = 3, scale bars = 20 mum. (D) Relative expression of Col6a5 mRNA in the ovaries and gonadal fat pads was decreased in DHT- treated mice after Col6a5 -LV injection; n = 6. The data are expressed as the mean +- SEM and were compared by Mann-Whitney U -test. (E) Histological images and mean size of ovarian stromal cells and gonadal adipocytes showing enhanced hypertrophy in DHT-treated mice after Col6a5 -LV injection, the cell area is based on the same measurement as in Figure 1F ; n = 6, scale bars = 20 mum. The data are expressed as the mean +- SEM and were compared by Mann-Whitney U -test. (F) Immunohistochemistry of PLIN2 (a marker of lipid droplets) in the ovaries showing decreased accumulation of lipid droplets in DHT-treated mice after Col6a5 -LV inje
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface Plasmon Resonance of COL6A5 polyclonal antibody (Product # PA5-70781). Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 µg/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333 nm peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
