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LearnPA5-47066
antibody from Invitrogen Antibodies
Targeting: IL36B
FIL1, FILI-(ETA), IL-1F8, IL-1H2, IL1-ETA, IL1F8, IL1H2, MGC126880, MGC126882
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
- Other assay [1]
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- Product number
- PA5-47066 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL36B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) IL-1 beta and rhIL-1F6 is observed and less than 1% cross-reactivity with rhIL-1 alpha and rhIL-18 is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization assay of recombinant human IL-36ß/IL-1F8 (aa 5-157) induces IL-8 secretion in human pre-adipocytes in a dose-dependent manner (orange line). IL-8 Secretion elicited by recombinant human IL-36ß/IL-1F8 (aa 5-157) (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-human IL-36ß/IL-1F8 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47066). The ND50 is typically 50-300 ng/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL36B in human pre-adipocytes. Samples were incubated in IL36B polyclonal antibody (Product # PA5-47066). Recombinant Human IL‚36β/IL‚1F8 (aa 5-157) induces IL-8 secretion in human pre-adipocytes in a dose-dependent manner (orange line). IL-8 Secretion elicited by Recombinant Human IL‚36β/IL‚1F8 (aa 5-157) (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‚36β/IL‚1F8 Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 50‚300 ng/mL.
