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- Antigen structure
- References [16]
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- Product number
- 14520-1-AP - Provider product page
- Provider
- Proteintech Group
- Product name
- UBC12 antibody
- Antibody type
- Polyclonal
- Description
- UBC12 antibody (Cat. #14520-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Neddylation Targets and Stabilizes NLRP3 to Augment Inflammasome-Mediated Colitis and Mood Disorder.
UBE2M promotes malignant phenotypes of prostate cancer through mediating NAA10 neddylation.
Regulation of UBC12 expression and protein neddylation by PINK1 suggests a primate-specific function.
Camk2n1 deficiency reduces the NaCl cotransporter activity through the CUL3/KLHL3/WNK4 complex in the kidney.
PRMT1-mediated methylation of UBE2m promoting calcium oxalate crystal-induced kidney injury by inhibiting fatty acid metabolism.
UBE2M inhibits neoplastic cell proliferation via MKK7-JNK-EGR1 axis in melanoma.
Neddylation signaling inactivation by tetracaine hydrochloride suppresses cell proliferation and alleviates vemurafenib-resistance of melanoma.
Proapoptotic effect of WS-299 induced by NOXA accumulation and NRF2-counterbalanced oxidative stress damage through targeting RBX1-UBE2M interaction in gastric cancers.
Neddylation activated TRIM25 desensitizes triple-negative breast cancer to paclitaxel via TFEB-mediated autophagy.
Mitoxantrone attenuates lipopolysaccharide-induced acute lung injury via inhibition of NEDD8 activating enzyme.
Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.
NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.
Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.
CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.
Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.
A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.
Gai W, Wu M, Wu A, Jing Z, Ye Z, Jin J, Zhang Y, Zhao M, Liu G, Wang X, Yang X, Dong J, Xu Y, Zhang J
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar;13(16):e05906
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar;13(16):e05906
UBE2M promotes malignant phenotypes of prostate cancer through mediating NAA10 neddylation.
Wang Q, Zhang J, Lin X, Shi R, Pan C, Hu Q, Xu L, Chen Q, Xue D, Yin J, Fu Q, Huang S
Journal of translational medicine 2026 Mar 19;24(1)
Journal of translational medicine 2026 Mar 19;24(1)
Regulation of UBC12 expression and protein neddylation by PINK1 suggests a primate-specific function.
Huang W, Xu T, Zhou GK, Li YX, Lin CZ, Chen XS, Zhang T, Jiang L, Liu YT, Xiong X, Liang XY, Guo XY, Li SH, Li XJ, Yang WL
Zoological research 2025 Nov 18;46(6):1488-1500
Zoological research 2025 Nov 18;46(6):1488-1500
Camk2n1 deficiency reduces the NaCl cotransporter activity through the CUL3/KLHL3/WNK4 complex in the kidney.
Zhang Y, Zhang Z, Jiang G, Zhang C
European journal of pharmacology 2025 Mar 5;990:177270
European journal of pharmacology 2025 Mar 5;990:177270
PRMT1-mediated methylation of UBE2m promoting calcium oxalate crystal-induced kidney injury by inhibiting fatty acid metabolism.
Yuan T, Ye Z, Mei S, Zhang M, Wu M, Lin F, Yu W, Li W, Zhou X, Cheng F
Cell death & disease 2025 Jul 31;16(1):579
Cell death & disease 2025 Jul 31;16(1):579
UBE2M inhibits neoplastic cell proliferation via MKK7-JNK-EGR1 axis in melanoma.
Xu L, Pan C, Wang Y, Ni L, Zeng P, Lin X, Lyu X, Huang S, Dang N
Journal of translational medicine 2025 Dec 8;24(1):52
Journal of translational medicine 2025 Dec 8;24(1):52
Neddylation signaling inactivation by tetracaine hydrochloride suppresses cell proliferation and alleviates vemurafenib-resistance of melanoma.
Huang X, Yi P, Gou W, Zhang R, Wu C, Liu L, He Y, Jiang X, Feng J
Cell biology and toxicology 2024 Sep 19;40(1):81
Cell biology and toxicology 2024 Sep 19;40(1):81
Proapoptotic effect of WS-299 induced by NOXA accumulation and NRF2-counterbalanced oxidative stress damage through targeting RBX1-UBE2M interaction in gastric cancers.
Ma T, Song Q, Cheng B, Guo E, Wang X, Li M, Dai M, Li S, Feng S, Yu B
Bioorganic chemistry 2024 Mar;144:107142
Bioorganic chemistry 2024 Mar;144:107142
Neddylation activated TRIM25 desensitizes triple-negative breast cancer to paclitaxel via TFEB-mediated autophagy.
Zheng B, Qian F, Wang X, Wang Y, Zhou B, Fang L
Journal of experimental & clinical cancer research : CR 2024 Jun 26;43(1):177
Journal of experimental & clinical cancer research : CR 2024 Jun 26;43(1):177
Mitoxantrone attenuates lipopolysaccharide-induced acute lung injury via inhibition of NEDD8 activating enzyme.
Liu H, Liu Y, Lin X, Fan J, Huang Z, Li A
International immunopharmacology 2024 Dec 25;143(Pt 3):113605
International immunopharmacology 2024 Dec 25;143(Pt 3):113605
Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.
Zhou W, Dong G, Gao G, He Z, Xu J, Aziz S, Ma L, Zhao W
Biochemistry and cell biology = Biochimie et biologie cellulaire 2022 Aug 1;100(4):309-324
Biochemistry and cell biology = Biochimie et biologie cellulaire 2022 Aug 1;100(4):309-324
NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.
Zhao X, Jiang L, Hu D, Tang Y, Zhao G, Du X, Luo S, Tang W
Experimental cell research 2021 Jun 15;403(2):112614
Experimental cell research 2021 Jun 15;403(2):112614
Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.
Fomicheva M, Macara IG
eLife 2020 Nov 13;9
eLife 2020 Nov 13;9
CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.
Li T, Wu S, Jia L, Cao W, Yao Y, Zhao G, Li H
Cancer biology & medicine 2020 May 15;17(2):357-370
Cancer biology & medicine 2020 May 15;17(2):357-370
Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.
Hong X, Li S, Li W, Xie M, Wei Z, Guo H, Wei W, Zhang S
Biochemical and biophysical research communications 2019 Jan 15;508(3):986-990
Biochemical and biophysical research communications 2019 Jan 15;508(3):986-990
A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.
Zhou H, Lu J, Liu L, Bernard D, Yang CY, Fernandez-Salas E, Chinnaswamy K, Layton S, Stuckey J, Yu Q, Zhou W, Pan Z, Sun Y, Wang S
Nature communications 2017 Oct 27;8(1):1150
Nature communications 2017 Oct 27;8(1):1150
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 14520-1-AP( UBC12 Antibody) at dilution of 1:600
- Other comments
- WB
