Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00009040-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009040-M01, RRID:AB_2211025
- Product name
- Mouse Monoclonal UBE2M/Ubc12 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. UBE2M - ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Application of an integrated physical and functional screening approach to identify inhibitors of the Wnt pathway.
Miller BW, Lau G, Grouios C, Mollica E, Barrios-Rodiles M, Liu Y, Datti A, Morris Q, Wrana JL, Attisano L
Molecular systems biology 2009;5:315
Molecular systems biology 2009;5:315
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - UBE2M monoclonal antibody (M01), clone 3C4 Analysis of UBE2M expression in Jurkat.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Detection limit for recombinant GST tagged UBE2M is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: UBE2M/Ubc12 Antibody (3C4) [H00009040-M01] - Analysis of monoclonal antibody to UBE2M on HeLa cell. Antibody concentration 10 ug/ml.