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- Antibody Data
- Antigen structure
- References [19]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 16113-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16113-1-AP, RRID:AB_2250974
- Product name
- MTHFD1L antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated MTHFD1L antibody (Cat. #16113-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references PKM2 diverts glycolytic flux in dependence on mitochondrial one-carbon cycle.
Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production.
Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells.
Mitochondria preserve an autarkic one-carbon cycle to confer growth-independent cancer cell migration and metastasis.
Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.
The SMYD3-MTHFD1L-formate metabolic regulatory axis mediates mitophagy to inhibit M1 polarization in macrophages.
Extracellular serine empowers epidermal proliferation and psoriasis-like symptoms.
Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.
MTHFD1L, A Folate Cycle Enzyme, Is Involved in Progression of Colorectal Cancer.
Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.
Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
Increased formate overflow is a hallmark of oxidative cancer.
Serine one-carbon catabolism with formate overflow.
Targeting MTHFD2 in acute myeloid leukemia.
Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.
Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.
Benzarti M, Neises L, Oudin A, Krötz C, Viry E, Gargiulo E, Pulido C, Schmoetten M, Pozdeev V, Lorenz NI, Ronellenfitsch MW, Sumpton D, Warmoes M, Jaeger C, Lesur A, Becker B, Moussay E, Paggetti J, Niclou SP, Letellier E, Meiser J
Cell reports 2024 Mar 26;43(3):113868
Cell reports 2024 Mar 26;43(3):113868
Mitochondrial One-Carbon Metabolism is Required for TGF-β-Induced Glycine Synthesis and Collagen Protein Production.
Meliton AY, Cetin-Atalay R, Tian Y, Szafran JCH, Shin KWD, Cho T, Sun KA, Woods PS, Shamaa OR, Chen B, Muir A, Mutlu GM, Hamanaka RB
bioRxiv : the preprint server for biology 2023 Nov 7;
bioRxiv : the preprint server for biology 2023 Nov 7;
Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
Gao Y, Zheng B, Xu S, Zhao Z, Liu W, Wang T, Yuan M, Sun X, Tan Y, Xu Q, Wu X
The Journal of biological chemistry 2023 Jul;299(7):104909
The Journal of biological chemistry 2023 Jul;299(7):104909
Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells.
Green AC, Marttila P, Kiweler N, Chalkiadaki C, Wiita E, Cookson V, Lesur A, Eiden K, Bernardin F, Vallin KSA, Borhade S, Long M, Ghahe EK, Jiménez-Alonso JJ, Jemth AS, Loseva O, Mortusewicz O, Meyers M, Viry E, Johansson AI, Hodek O, Homan E, Bonagas N, Ramos L, Sandberg L, Frödin M, Moussay E, Slipicevic A, Letellier E, Paggetti J, Sørensen CS, Helleday T, Henriksson M, Meiser J
Nature metabolism 2023 Apr;5(4):642-659
Nature metabolism 2023 Apr;5(4):642-659
Mitochondria preserve an autarkic one-carbon cycle to confer growth-independent cancer cell migration and metastasis.
Kiweler N, Delbrouck C, Pozdeev VI, Neises L, Soriano-Baguet L, Eiden K, Xian F, Benzarti M, Haase L, Koncina E, Schmoetten M, Jaeger C, Noman MZ, Vazquez A, Janji B, Dittmar G, Brenner D, Letellier E, Meiser J
Nature communications 2022 May 16;13(1):2699
Nature communications 2022 May 16;13(1):2699
Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.
Soflaee MH, Kesavan R, Sahu U, Tasdogan A, Villa E, Djabari Z, Cai F, Tran DH, Vu HS, Ali ES, Rion H, O'Hara BP, Kelekar S, Hallett JH, Martin M, Mathews TP, Gao P, Asara JM, Manning BD, Ben-Sahra I, Hoxhaj G
Nature communications 2022 May 16;13(1):2698
Nature communications 2022 May 16;13(1):2698
The SMYD3-MTHFD1L-formate metabolic regulatory axis mediates mitophagy to inhibit M1 polarization in macrophages.
Zhu W, Wang C, Xue L, Liu L, Yang X, Liu Z, Zhang S, Luo D
International immunopharmacology 2022 Dec;113(Pt A):109352
International immunopharmacology 2022 Dec;113(Pt A):109352
Extracellular serine empowers epidermal proliferation and psoriasis-like symptoms.
Cappello A, Mancini M, Madonna S, Rinaldo S, Paone A, Scarponi C, Belardo A, Zolla L, Zuccotti A, Panatta E, Pallotta S, Annicchiarico-Petruzzelli M, Albanesi C, Cutruzzolà F, Wang L, Jia W, Melino G, Candi E
Science advances 2022 Dec 16;8(50):eabm7902
Science advances 2022 Dec 16;8(50):eabm7902
Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.
Lee WD, Pirona AC, Sarvin B, Stern A, Nevo-Dinur K, Besser E, Sarvin N, Lagziel S, Mukha D, Raz S, Aizenshtein E, Shlomi T
Cell metabolism 2021 Jan 5;33(1):190-198.e6
Cell metabolism 2021 Jan 5;33(1):190-198.e6
Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.
Cui L, Zhao X, Jin Z, Wang H, Yang SF, Hu S
Journal of pineal research 2021 Dec;71(4):e12767
Journal of pineal research 2021 Dec;71(4):e12767
Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.
Liu YT, Huang X, Nguyen D, Shammas MK, Wu BP, Dombi E, Springer DA, Poulton J, Sekine S, Narendra DP
Human molecular genetics 2020 Jun 3;29(9):1547-1567
Human molecular genetics 2020 Jun 3;29(9):1547-1567
MTHFD1L, A Folate Cycle Enzyme, Is Involved in Progression of Colorectal Cancer.
Agarwal S, Behring M, Hale K, Al Diffalha S, Wang K, Manne U, Varambally S
Translational oncology 2019 Nov;12(11):1461-1467
Translational oncology 2019 Nov;12(11):1461-1467
Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.
Forsström S, Jackson CB, Carroll CJ, Kuronen M, Pirinen E, Pradhan S, Marmyleva A, Auranen M, Kleine IM, Khan NA, Roivainen A, Marjamäki P, Liljenbäck H, Wang L, Battersby BJ, Richter U, Velagapudi V, Nikkanen J, Euro L, Suomalainen A
Cell metabolism 2019 Dec 3;30(6):1040-1054.e7
Cell metabolism 2019 Dec 3;30(6):1040-1054.e7
Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.
Koufaris C, Nilsson R
Cancer & metabolism 2018;6:12
Cancer & metabolism 2018;6:12
Increased formate overflow is a hallmark of oxidative cancer.
Meiser J, Schuster A, Pietzke M, Vande Voorde J, Athineos D, Oizel K, Burgos-Barragan G, Wit N, Dhayade S, Morton JP, Dornier E, Sumpton D, Mackay GM, Blyth K, Patel KJ, Niclou SP, Vazquez A
Nature communications 2018 Apr 10;9(1):1368
Nature communications 2018 Apr 10;9(1):1368
Serine one-carbon catabolism with formate overflow.
Meiser J, Tumanov S, Maddocks O, Labuschagne CF, Athineos D, Van Den Broek N, Mackay GM, Gottlieb E, Blyth K, Vousden K, Kamphorst JJ, Vazquez A
Science advances 2016 Oct;2(10):e1601273
Science advances 2016 Oct;2(10):e1601273
Targeting MTHFD2 in acute myeloid leukemia.
Pikman Y, Puissant A, Alexe G, Furman A, Chen LM, Frumm SM, Ross L, Fenouille N, Bassil CF, Lewis CA, Ramos A, Gould J, Stone RM, DeAngelo DJ, Galinsky I, Clish CB, Kung AL, Hemann MT, Vander Heiden MG, Banerji V, Stegmaier K
The Journal of experimental medicine 2016 Jun 27;213(7):1285-306
The Journal of experimental medicine 2016 Jun 27;213(7):1285-306
Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.
Nikkanen J, Forsström S, Euro L, Paetau I, Kohnz RA, Wang L, Chilov D, Viinamäki J, Roivainen A, Marjamäki P, Liljenbäck H, Ahola S, Buzkova J, Terzioglu M, Khan NA, Pirnes-Karhu S, Paetau A, Lönnqvist T, Sajantila A, Isohanni P, Tyynismaa H, Nomura DK, Battersby BJ, Velagapudi V, Carroll CJ, Suomalainen A
Cell metabolism 2016 Apr 12;23(4):635-48
Cell metabolism 2016 Apr 12;23(4):635-48
Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.
Thomas A, Stevens AP, Klein MS, Hellerbrand C, Dettmer K, Gronwald W, Oefner PJ, Reinders J
Proteomics 2012 May;12(9):1437-51
Proteomics 2012 May;12(9):1437-51
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16113-1-AP(MTHFD1L antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 16113-1-AP(MTHFD1L antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MTHFD1L antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MTHFD1L. This antibody recognizes human,mouse,rat antigen. The MTHFD1L antibody has been validated for the following applications: ELISA, WB, IHC analysis.
