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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX108953 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX108953, RRID:AB_1951244
- Product name
- PI3 kinase p150 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling.
Nemazanyy I, Montagnac G, Russell RC, Morzyglod L, Burnol AF, Guan KL, Pende M, Panasyuk G
Nature communications 2015 Sep 21;6:8283
Nature communications 2015 Sep 21;6:8283
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: Molt-4 (GTX27912)B: Raji7.5% SDS PAGEGTX108953 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with PI3-Kinase p150 antibody [C1C3] (GTX108953) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PI3-Kinase p150 protein from HeLa whole cell extracts using 5 £gg of PI3-Kinase p150 antibody [C1C3] (GTX108953).Western blot analysis was performed using PI3-Kinase p150 antibody [C1C3] (GTX108953).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
