14346-1-AP

antibody from Proteintech Group

Targeting: USP1

Provider product page for 14346-1-AP

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [46]
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Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 14346-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#14346-1-AP, RRID:AB_2214314
Product name: USP1 antibody
Antibody type: Polyclonal
Description: KD/KO validated USP1 antibody (Cat. #14346-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, IP, WB,ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

USP1 protein structure - 14346-1-AP shown in red.

Submitted references A Novel Insight into Chronic Pancreatitis Pathogenesis: the USP1/ITGB5 Axis-Mediated Stellate Cell Activation.

Wang D, Shi Y, Lu J, Bao W, Wang N, Shen Q, Lin Z, Qian L, Zhu C
Inflammation 2026 Mar 19;49(1)

USP1 driven mitotic dysregulation and PLK1 stabilization confer Lenvatinib resistance in hepatocellular carcinoma.

Qiang Y, Bian S, Tong Y, Chen W, Tang Z, Dai C, Liu M, Xu B, Jiang L, Ma K, He X, Li J, Xiao M, Zheng W
Journal of experimental & clinical cancer research : CR 2026 Mar 13;45(1)

TAZ alleviates ischemic stroke injury by activating Foxo1: Usp1-mediated deubiquitination is the pivotal mechanism.

Xin Y, Sun Y, Pan E, Li M, Chen H, Xu Y, Zhang B, Shi Y, Dong J, Zhao P
Free radical biology & medicine 2026 Jul;250:548-563

The USP1-WDR48 deubiquitinase complex functions as a molecular switch regulating tumor-associated macrophage activation and anti-tumor response.

Han D, Wang L, Jiang S, Su P, Chen B, Zhao W, Chen T, Zhang N, Wang X, Liang Y, Li Y, Li C, Chen X, Luo D, Yang Q
Cell death and differentiation 2026 Jan;33(1):92-110

MGMT upregulation mediates Temozolomide resistance conferred USP5 dependency.

Bhardwaj S, Sanjay, Sharma D, Taneja L, Yadav BK, Yadav AK
Scientific reports 2026 Jan 24;16(1):6118

RPA exhaustion activates SLFN11 to eliminate cells with heightened replication stress.

Stanage TH, Li S, Segura-Bayona S, Idilli AI, Millar R, Hewitt G, Boulton SJ
Nature cell biology 2026 Feb;28(2):240-254

USP1 dependent stabilization of LTA4H drives hepatocellular carcinoma angiogenesis.

Xu B, Wang Y, Lin Z, Chen W, Liu M, Tang Z, Ma K, Jiang L, Zheng W, Bian S
Biochimica et biophysica acta. Molecular basis of disease 2026 Apr 25;1872(6):168270

USP1 promotes pancreatic cancer progression and autophagy by deubiquitinating ATG14.

Li L, Fan Z, Liu M, Dong H, Li J, Li Y, Song Z, Liu Y, Zhang Z, Gu X, Zhang T
The Journal of biological chemistry 2025 Mar;301(3):108190

An integrated proteomic portrait of prostate cancer progression.

Zhang J, Rivera KD, Bossi D, Gianfanti F, Nicastri S, Gomes D, Matkovic M, Coazzoli M, Mosole S, Costanzo F, Vallerga A, Ceserani V, Cavalli M, Virshup M, Burt RA, Bolis M, Ruthishauser D, Stathis A, Moch H, Bubendorf L, Cavalli A, Corey E, Wang Y, Mani DR, Carr SA, Udeshi N, Theurillat JP
Cell reports 2025 Jun 24;44(6):115828

Survivin Is a Central Mediator of Cell Proliferation in HPV-Negative Head and Neck Squamous Cell Carcinoma.

Zhu J, An J, Hu E, Rosenblatt G, Berner G, Roy A, Kawachi N, Shrivastava N, Mehta V, Segall JE, Prystowsky MB, Ow TJ
Cancers 2025 Aug 31;17(17)

Ubiquitin-specific protease 1 facilitates hepatocellular carcinoma progression by modulating mitochondrial fission and metabolic reprogramming via cyclin-dependent kinase 5 stabilization.

Bian S, Ni W, Zhou L, Tong Y, Dai C, Zhao X, Qiang Y, Gao J, Xiao Y, Liu W, Chen C, Lin S, Gong J, Zhao S, Chen Y, Lin Z, Liu D, Zhao H, Zheng W
Cell death and differentiation 2024 Sep;31(9):1202-1218

CD98hc promotes drug resistance in extranodal natural killer/T cell lymphoma through tumor cell-derived small extracellular vesicles.

Liao L, Yang P, Zhang W, Yu S, Jing H, Zheng X
Science signaling 2024 Sep 10;17(853):eadf9388

Ubiquitin-Specific Protease 1 Promotes Bladder Cancer Progression by Stabilizing c-MYC.

Zhang X, Peng P, Bao LW, Zhang AQ, Yu B, Li T, Lei J, Zhang HH, Li SZ
Cells 2024 Oct 30;13(21)

USP9X-mediated REV1 deubiquitination promotes lung cancer radioresistance via the action of REV1 as a Rad18 molecular scaffold for cystathionine γ-lyase.

Chen Y, Feng X, Wu Z, Yang Y, Rao X, Meng R, Zhang S, Dong X, Xu S, Wu G, Jie X
Journal of biomedical science 2024 May 28;31(1):55

The P53-P21-RB1 pathway promotes BRD4 degradation in liver cancer through USP1.

Li N, Zhang E, Li Z, Lv S, Zhao X, Ke Q, Zou Q, Li W, Wang Y, Guo H, Song T, Sun L
The Journal of biological chemistry 2024 Mar;300(3):105707

Stress granule-localized USP8 potentiates cGAS-mediated type I interferonopathies through deubiquitination of DDX3X.

Zhang X, Han L, Hou J, Yang H, Xu H, Li G, Shu Q, Zhu D, Zheng Y, Gao C
Cell reports 2024 Jun 25;43(6):114248

Deubiquitinase USP1 regulates sarbecovirus ORF6 protein function.

Gao W, Wang L, Cui W, Wang H, Huang G, Li Z, Li G, Zhang W
Journal of virology 2024 Jan 23;98(1):e0143723

Multi-regulatory potency of USP1 on inflammasome components promotes pyroptosis in thyroid follicular cells and contributes to the progression of Hashimoto's thyroiditis.

Zhao X, Ni W, Zheng W, Ni W, Sun C, Gu Y, Gu Z
Molecular medicine (Cambridge, Mass.) 2024 Aug 12;30(1):121

Variety in the USP deubiquitinase catalytic mechanism.

Keijzer N, Priyanka A, Stijf-Bultsma Y, Fish A, Gersch M, Sixma TK
Life science alliance 2024 Apr;7(4)

CDK9-55 guides the anaphase-promoting complex/cyclosome (APC/C) in choosing the DNA repair pathway choice.

Alfano L, Iannuzzi CA, Barone D, Forte IM, Ragosta MC, Cuomo M, Mazzarotti G, Dell'Aquila M, Altieri A, Caporaso A, Roma C, Marra L, Boffo S, Indovina P, De Laurentiis M, Giordano A
Oncogene 2024 Apr;43(17):1263-1273

The deubiquitinase cofactor UAF1 interacts with USP1 and plays an essential role in spermiogenesis.

Wang Z, Li T, Liu D, Li M, Liu S, Yu X, Li H, Song H, Zhao W, Liu Z, Chen X, Lu G, Chen ZJ, Huang T, Liu H
iScience 2024 Apr 19;27(4):109456

Interaction of TAGLN and USP1 promotes ZEB1 ubiquitination degradation in UV-induced skin photoaging.

Li Y, Huang X, Jin J, Zhang H, Yang K, Han J, Lv Y, Sun Y, Yao C, Lin T, Zhu C, Liu H
Cell & bioscience 2023 May 6;13(1):80

The promotive role of USP1 inhibition in coordinating osteogenic differentiation and fracture healing during nonunion.

Huang J, Zhou H, He L, Zhong L, Zhou D, Yin Z
Journal of orthopaedic surgery and research 2023 Mar 2;18(1):152

Detection of endogenous translesion DNA synthesis in single mammalian cells.

Egger T, Aze A, Maiorano D
Cell reports methods 2023 Jun 26;3(6):100501

Phosphorylation by ATR triggers FANCD2 chromatin loading and activates the Fanconi anemia pathway.

Kupculak M, Bai F, Luo Q, Yoshikawa Y, Lopez-Martinez D, Xu H, Uphoff S, Cohn MA
Cell reports 2023 Jul 25;42(7):112721

USP1 promotes the aerobic glycolysis and progression of T-cell acute lymphoblastic leukemia via PLK1/LDHA axis.

Liu S, Xiang Y, Wang B, Gao C, Chen Z, Xie S, Wu J, Liu Y, Zhao X, Yang C, Yue Z, Wang L, Wen X, Zhang R, Zhang F, Xu H, Zhai X, Zheng H, Zhang H, Qian M
Blood advances 2023 Jul 11;7(13):3099-3112

CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells.

Tyagi A, Kaushal K, Chandrasekaran AP, Sarodaya N, Das S, Park CH, Hong SH, Kim KS, Ramakrishna S
Theranostics 2022;12(13):5949-5970

H3F3A K27M Mutation Promotes the Infiltrative Growth of High-Grade Glioma in Adults by Activating β-Catenin/USP1 Signaling.

Sun Z, Zhu Y, Feng X, Liu X, Zhou K, Wang Q, Zhang H, Shi H
Cancers 2022 Oct 3;14(19)

Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway.

Hunter JE, Campbell AE, Hannaway NL, Kerridge S, Luli S, Butterworth JA, Sellier H, Mukherjee R, Dhillon N, Sudhindar PD, Shukla R, Brownridge PJ, Bell HL, Coxhead J, Taylor L, Leary P, Hasoon MSR, Collins I, Garrett MD, Eyers CE, Perkins ND
The Biochemical journal 2022 Oct 14;479(19):2063-2086

HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway.

Huang Z, Chen Y, Chen R, Zhou B, Wang Y, Hong L, Wang Y, Wang J, Xu X, Huang Z, Chen W
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2022 Nov 16;10(1):e2202437

Inhibition of USP1 activates ER stress through Ubi-protein aggregation to induce autophagy and apoptosis in HCC.

Wang L, Hu T, Shen Z, Zheng Y, Geng Q, Li L, Sha B, Li M, Sun Y, Guo Y, Xue W, Xuan D, Chen P, Zhao J
Cell death & disease 2022 Nov 10;13(11):951

Ubiquitin-specific protease 1 acts as an oncogene and promotes lenvatinib efficacy in hepatocellular carcinoma by stabilizing c-kit.

Chen Z, Ma Y, Guo Z, Song D, Chen Z, Sun M
Annals of hepatology 2022 Mar-Apr;27(2):100669

FANCD2 promotes the malignant behavior of endometrial cancer cells and its prognostic value.

Zheng C, Ren Z, Chen H, Yuan X, Suye S, Yin H, Zhou Z, Fu C
Experimental cell research 2022 Dec 15;421(2):113388

USP1 Inhibits NF-κB/NLRP3 Induced Pyroptosis through TRAF6 in Osteoblastic MC3T3-E1 Cells.

Sun D, Peng Y, Ge S, Fu Q
Journal of musculoskeletal & neuronal interactions 2022 Dec 1;22(4):536-545

USP1 Promotes GC Metastasis via Stabilizing ID2.

Li N, Wu L, Zuo X, Luo H, Sheng Y, Yan J
Disease markers 2021;2021:3771990

USP1-WDR48 deubiquitinase complex enhances TGF-β induced epithelial-mesenchymal transition of TNBC cells via stabilizing TAK1.

Han D, Wang L, Chen B, Zhao W, Liang Y, Li Y, Zhang H, Liu Y, Wang X, Chen T, Li C, Song X, Luo D, Li Z, Yang Q
Cell cycle (Georgetown, Tex.) 2021 Feb;20(3):320-331

USP1 Maintains the Survival of Liver Circulating Tumor Cells by Deubiquitinating and Stabilizing TBLR1.

Li Y, Xu Y, Gao C, Sun Y, Zhou K, Wang P, Cheng J, Guo W, Ya C, Fan J, Yang X
Frontiers in oncology 2020;10:554809

Comprehensive analysis of ubiquitin-specific protease 1 reveals its importance in hepatocellular carcinoma.

Zhao Y, Xue C, Xie Z, Ouyang X, Li L
Cell proliferation 2020 Oct;53(10):e12908

hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma.

Herviou P, Le Bras M, Dumas L, Hieblot C, Gilhodes J, Cioci G, Hugnot JP, Ameadan A, Guillonneau F, Dassi E, Cammas A, Millevoi S
Nature communications 2020 May 27;11(1):2661

USP1 deubiquitinates Akt to inhibit PI3K-Akt-FoxO signaling in muscle during prolonged starvation.

Goldbraikh D, Neufeld D, Eid-Mutlak Y, Lasry I, Gilda JE, Parnis A, Cohen S
EMBO reports 2020 Apr 3;21(4):e48791

USP22 Deubiquitinates CD274 to Suppress Anticancer Immunity.

Huang X, Zhang Q, Lou Y, Wang J, Zhao X, Wang L, Zhang X, Li S, Zhao Y, Chen Q, Liang T, Bai X
Cancer immunology research 2019 Oct;7(10):1580-1590

USP1 inhibition destabilizes KPNA2 and suppresses breast cancer metastasis.

Ma A, Tang M, Zhang L, Wang B, Yang Z, Liu Y, Xu G, Wu L, Jing T, Xu X, Yang S, Liu Y
Oncogene 2019 Mar;38(13):2405-2419

The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.

Ha K, Ma C, Lin H, Tang L, Lian Z, Zhao F, Li JM, Zhen B, Pei H, Han S, Malumbres M, Jin J, Chen H, Zhao Y, Zhu Q, Zhang P
Nature communications 2017 Jun 12;8:15751

USP22 acts as an oncogene by regulating the stability of cyclooxygenase-2 in non-small cell lung cancer.

Xiao H, Tian Y, Yang Y, Hu F, Xie X, Mei J, Ding F
Biochemical and biophysical research communications 2015 May 8;460(3):703-8

A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.

Liang Q, Dexheimer TS, Zhang P, Rosenthal AS, Villamil MA, You C, Zhang Q, Chen J, Ott CA, Sun H, Luci DK, Yuan B, Simeonov A, Jadhav A, Xiao H, Wang Y, Maloney DJ, Zhuang Z
Nature chemical biology 2014 Apr;10(4):298-304

Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.

Ciccia A, Nimonkar AV, Hu Y, Hajdu I, Achar YJ, Izhar L, Petit SA, Adamson B, Yoon JC, Kowalczykowski SC, Livingston DM, Haracska L, Elledge SJ
Molecular cell 2012 Aug 10;47(3):396-409

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 14346-1-AP(USP1 antibody) at dilution of 1:300
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The USP1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human USP1. This antibody recognizes human,mouse,rat antigen. The USP1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 14346-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human cervical cancer using 14346-1-AP(USP1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue