A301-699A

antibody from Invitrogen Antibodies

Targeting: USP1

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-699A

Provider

Invitrogen Antibodies

Product name

USP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human USP1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- USP1 (Cat. No. A301-699A Lot2) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody A301-699A (lot A301-699A-2) used for IP at 6 µg per reaction. USP1 was also immunoprecipitated by rabbit anti-USP1 antibodies A301-698A and A301-700A. For blotting immunoprecipitated USP1, A301-699A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.