Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-88117 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-88117, RRID:AB_1218320
- Product name
- Rabbit Polyclonal USP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Regulation of the Fanconi anemia pathway by a SUMO-like delivery network.
The USP1/UAF1 complex promotes double-strand break repair through homologous recombination.
Yang K, Moldovan GL, Vinciguerra P, Murai J, Takeda S, D'Andrea AD
Genes & development 2011 Sep 1;25(17):1847-58
Genes & development 2011 Sep 1;25(17):1847-58
The USP1/UAF1 complex promotes double-strand break repair through homologous recombination.
Murai J, Yang K, Dejsuphong D, Hirota K, Takeda S, D'Andrea AD
Molecular and cellular biology 2011 Jun;31(12):2462-9
Molecular and cellular biology 2011 Jun;31(12):2462-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: USP1 Antibody [NB100-88117] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-88117 used for IP at 3 mcg/mg lysate. USP1 was also immunoprecipitated by NB100-88116 and NB100-88118, which recognize other epitopes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: USP1 Antibody [NB100-88117] - Detection of human USP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-USP1 antibody NB100-88117 used for IP at 6 ug per reaction. USP1 was also immunoprecipitated by rabbit anti-USP1 antibodies NB100-88116 and NB100-88118. For blotting immunoprecipitated USP1, NB100-88117 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117] - FFPE section of human thyroid carcinoma. Affinity purified rabbit anti-USP1 used at a dilution of 1:250.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: USP1 Antibody [NB100-88117] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- USP1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB