Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 16584-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#16584-1-AP, RRID:AB_10639509
- Product name
- MRPL3 antibody
- Antibody type
- Polyclonal
- Description
- MRPL3 antibody (Cat. #16584-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Protocol to study human mitochondrial ribosome using quantitative density gradient analysis by mass spectrometry and complexome profiling analysis.
Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.
Exome sequencing identifies MRPL3 mutation in mitochondrial cardiomyopathy.
Páleníková P, Minczuk M, Rebelo-Guiomar P
STAR protocols 2023 Dec 15;4(4):102605
STAR protocols 2023 Dec 15;4(4):102605
Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors.
Dennerlein S, Poerschke S, Oeljeklaus S, Wang C, Richter-Dennerlein R, Sattmann J, Bauermeister D, Hanitsch E, Stoldt S, Langer T, Jakobs S, Warscheid B, Rehling P
eLife 2021 Dec 31;10
eLife 2021 Dec 31;10
The human Obg protein GTPBP10 is involved in mitoribosomal biogenesis.
Lavdovskaia E, Kolander E, Steube E, Mai MM, Urlaub H, Richter-Dennerlein R
Nucleic acids research 2018 Sep 19;46(16):8471-8482
Nucleic acids research 2018 Sep 19;46(16):8471-8482
Mutations in mitochondrial ribosomal protein MRPL12 leads to growth retardation, neurological deterioration and mitochondrial translation deficiency.
Serre V, Rozanska A, Beinat M, Chretien D, Boddaert N, Munnich A, Rötig A, Chrzanowska-Lightowlers ZM
Biochimica et biophysica acta 2013 Aug;1832(8):1304-12
Biochimica et biophysica acta 2013 Aug;1832(8):1304-12
Exome sequencing identifies MRPL3 mutation in mitochondrial cardiomyopathy.
Galmiche L, Serre V, Beinat M, Assouline Z, Lebre AS, Chretien D, Nietschke P, Benes V, Boddaert N, Sidi D, Brunelle F, Rio M, Munnich A, Rötig A
Human mutation 2011 Nov;32(11):1225-31
Human mutation 2011 Nov;32(11):1225-31
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16584-1-AP(MRPL3 antibody) at dilution of 1:1000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human cervical cancer using 16584-1-AP(MRPL3 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MRPL3 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MRPL3. This antibody recognizes human,mouse,rat antigen. The MRPL3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.