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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [2]
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- Product number
- 10477-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHMP2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MDLLFGRRK TPEELLRQNQ RALNRAMREL DRERQKLETQ EKKIIADIKK MAKQGQMDAV RIMAKDLVRT RRYVRKFVLM RANIQAVSLK IQTLKSNNSM AQAMKGVTKA MGTMNRQLKL PQIQKIMMEF ERQAEIMDMK EEMMNDAIDD AMGDEEDEEE SDAVVSQVLD ELGLSLTDEL SNLPSTGGSL SVAAGGKKAE AAASALADAD ADLEERLKNL R (1-220 aa encoded by BC002502)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.19 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 10477-1-AP (CHMP2A Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 10477-1-AP ( CHMP2A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
