Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG55694 - Provider product page
- Provider
- Arigo
- Product name
- anti-ROR1 antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein around aa. 112-399 of Human ROR1.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of K562 cell line, Human lung, Mouse kidney, Mouse heart tissue (from left to right) stained with ARG55694 anti-ROR1 antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of K562 cell line, Human lung, Mouse kidney tissue lysate (from left to right) stained with ARG55694 anti-ROR1 antibody at 1:2000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 1) Human lung lysates, 2) K562 whole cell lysates, 3) Mouse kidney lysates stained with ARG55694 anti-ROR1 antibody at 1:2000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG55694 anti-ROR1 antibody.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: NCI-H460 cells stained with ARG55694 anti-ROR1 antibody (bottom histogram) or without primary antibody control (top histogram), followed by incubation with FITC labelled secondary antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: A549 cells stained with ARG55694 anti-ROR1 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor? 488 labelled secondary antibody.