Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ARG55693 - Provider product page
- Provider
- Arigo
- Product name
- anti-EN1 / Engrailed 1 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 1-30 (N-terminus) of Human EN1 / Engrailed 1.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 293, SH-SY5Y, NCI-H460 cell line (from left to right) stained with ARG55693 anti-EN1 / Engrailed 1 antibody at 1:1000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 20 µg of 1) NCI-H460, 2) SH-SY5Y, 3) PC-3, 4) Jurkat, and 5) 293T whole cell lysates stained with ARG55693 anti-EN1 / Engrailed 1 antibody at 1:2000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: U251 cell stained with ARG55693 anti-EN1 / Engrailed 1 antibody (green) at 1:25 dilution, 1 h at 37°C. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARG55693 anti-EN1 / Engrailed 1 antibody. Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). EN1 immunoreactivity is localized to Nucleus significantly.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded Human lung carcinoma tissue stained with ARG55693 anti-EN1 / Engrailed 1 antibody.