PAB2058

antibody from Abnova Corporation

Targeting: EN1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2058

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2058, RRID:AB_1574669

Product name

EN1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of EN1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Engrailed-1 negatively regulates beta-catenin transcriptional activity by destabilizing beta-catenin via a glycogen synthase kinase-3beta-independent pathway.

Bachar-Dahan L, Goltzmann J, Yaniv A, Gazit A
Molecular biology of the cell 2006 Jun;17(6):2572-80

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Regional assignment of the human homeobox-containing gene EN1 to chromosome 2q13-q21.

Köhler A, Logan C, Joyner AL, Muenke M
Genomics 1993 Jan;15(1):233-5

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of EN1 polyclonal antibody (Cat # PAB2058) in NCI-H460 cell line lysates (35 ug/lane). EN1 (arrow) was detected using the purified Polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 polyclonal antibody (Cat # PAB2058) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)