F54331

antibody from NSJ Bioreagents

Targeting: EN1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54331

Provider

NSJ Bioreagents

Product name

Engrailed 1 Antibody / EN1

Antibody type

Polyclonal

Description

This highly specific Engrailed 1 antibody is suitable for use in Immunofluorescence/Western blot applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Engrailed 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) U-87 MG, 2) U-2 OS, 3) SH-SY5Y, 4) 293T, 5) HepG2 and 6) mouse brain lysate with Engrailed 1 antibody. Predicted molecular weight ~40 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human U-2 OS cells with Engrailed 1 antibody (green) and anti-Actin (red).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human U-251 cells with Engrailed 1 antibody (green) and anti-Actin (red).