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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- PA5-14149 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Generation of human induced pluripotent stem cell-derived cerebral organoids for cellular and molecular characterization.
Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis.
Anastasaki C, Wilson AF, Chen AS, Wegscheid ML, Gutmann DH
STAR protocols 2022 Mar 18;3(1):101173
STAR protocols 2022 Mar 18;3(1):101173
Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis.
Wegscheid ML, Anastasaki C, Hartigan KA, Cobb OM, Papke JB, Traber JN, Morris SM, Gutmann DH
Cell reports 2021 Jul 6;36(1):109315
Cell reports 2021 Jul 6;36(1):109315
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of U251 cells stained with an EN1 polyclonal antibody (Product # PA5-14149). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with an EN1 polyclonal antibody (Product # PA5-14149) (1:25, 1 hr at 37ºC). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37ºC). Actin filaments have been labeled with dye-conjugated phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of EN1 in U251 cells. Samples were incubated with EN1 polyclonal antibody (Product # PA5-14149) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). EN1 immunoreactivity is localized to Nucleus significantly.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of EN1 in U-2 OS (Human Sarcoma cell line) cells. Samples were incubated with EN1 polyclonal antibody (Product # PA5-14149) using a dilution of 0.0590277777777778 followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG at a dilution of 1:400 (green). Cells were 4% paraformaldehyde-fixed and 0.1% Triton X-100 permeabilized. Immunofluorescence image showing nucleus and nucleoli staining on U-2 OS cell line. Cytoplasmic actin is detected with Alexa Fluor® 555 conjugated with Phalloidin at 1:100 dilution (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EN1 in formalin-fixed and paraffin-embedded human lung carcinoma tissue. Samples were incubated with EN1 polyclonal antibody (Product # PA5-14149) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of EN1 in paraformaldehyde-fixed paraffin embedded human tonsil tissue sections . Samples were incubated with EN1 polyclonal antibody (Product # PA5-14149) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6).
