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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-89139 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DBI Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MWGDLWLLPP ASANPGTGTE AEFEKAAEEV RHLKTKPSDE EMLFIYGHYK QATVGDINTE RPGMLDFTGK AKWDAWNELK GTSKEDAMKA YINKVEELKK KYGI; Positive Samples: HT-29, THP-1, MCF-7, NCI-H460, A673, U-937; Cellular Location: Endoplasmic reticulum, Golgi apparatus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DBI was performed in MCF-7 cells using DBI Polyclonal Antibody (Product # PA5-89139). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DBI in paraffin-embedded rat ovary. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DBI in paraffin-embedded rat liver. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
