PA5-89139

antibody from Invitrogen Antibodies

Targeting: DBI ACBD1, ACBP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-89139

Provider

Invitrogen Antibodies

Product name

DBI Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MWGDLWLLPP ASANPGTGTE AEFEKAAEEV RHLKTKPSDE EMLFIYGHYK QATVGDINTE RPGMLDFTGK AKWDAWNELK GTSKEDAMKA YINKVEELKK KYGI; Positive Samples: HT-29, THP-1, MCF-7, NCI-H460, A673, U-937; Cellular Location: Endoplasmic reticulum, Golgi apparatus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.84 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DBI in extracts of various cell lines. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DBI in extracts of various cell lines. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DBI was performed in MCF-7 cells using DBI Polyclonal Antibody (Product # PA5-89139). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DBI in U2OS cells. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DBI in A-549 cells. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DBI in NIH/3T3 cells. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DBI in PC-12 cells. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of DBI in 300 μg extracts of Hela cells. Samples were precipitated with 3 μg DBI Polyclonal antibody (Product # PA5-89139). Western blot was performed from the immunoprecipitate using DBI Polyclonal antibody (Product # PA5-89139) at a dilution of 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DBI in paraffin-embedded rat ovary. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DBI in paraffin-embedded rat liver. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DBI in paraffin-embedded rat liver. Samples were incubated with DBI Polyclonal antibody (Product # PA5-89139) using a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.