20966-1-AP

antibody from Proteintech Group

Targeting: LEPR CD295, OBR

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

20966-1-AP

Provider

Proteintech Group

Product name

LEPR antibody

Antibody type

Polyclonal

Description

KD/KO validated LEPR antibody (Cat. #20966-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

CGRP Enhances the Regeneration of Bone Defects by Regulating Bone Marrow Mesenchymal Stem Cells Through Promoting ANGPTL4 Secretion by Bone Blood Vessels.

Lu Q, Zheng Q, Zhou Z, Chen Y, Chen Y, Chen W, Wang J, Guo R, Wu R, Chen L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar;13(14):e22295

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Effects of different pre-conditioning exercise on leptin synthesis and its downstream signalling pathway in T2DM rats.

Lin S, Hu Y, Ding S, Hu Y
Iranian journal of basic medical sciences 2025;28(1):31-37

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Evaluation of bone regenerative and bioactive potential of β-TCP/PLLA/PGA nanobiomaterial in conjunction with concentrated conditioned media from rapidly expanding clone (REC) of mesenchymal stem cells: A pilot animal study.

Shijirbold A, Ramanathan M, Fujioka-Kobayashi M, Sankepally N, Matsuda Y, Matsuzaki Y, Fujita Y, Kanno T
PloS one 2025;20(10):e0332531

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Modulation of senescent Lepr(+) skeletal stem cells via suppression of leptin-induced STAT3‒FGF7 axis activation alleviates abnormal subchondral bone remodeling and osteoarthritis progression.

Yu FH, Yin BF, Liu MY, Zhang WJ, Zhao ZD, Wang L, Li XT, Li PL, Li ZL, Xu RX, Ding L, Zhu H
Stem cell research & therapy 2025 May 5;16(1):227

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Directional Freeze-Casting Cryogel Loaded with Quaternized Chitosan Modified Gallium Metal-Organic Frameworks to Capture and Eradicate the Resistant Bacteria for Guided Regeneration in Infected Bone Defects.

Liu Y, Yuan K, Lin Y, Yang Y, Kong W, Shan J, Niu H, Kong W, Li F, Yue X, Du Y, Liang Y, Chang H, Yu Z, Wang J, Yang G, Cao L, Huang K, Yang S, Tang T
Advanced materials (Deerfield Beach, Fla.) 2025 Mar;37(9):e2414437

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A positive feedback loop of OTUD1 and c-Jun driven by leptin expedites stemness maintenance in ovarian cancer.

Wang J, Yang F, Chen Y, Xing Y, Huang J, Cao J, Xiong J, Liu Y, Zhao Q, Luo M, Xiong J, Fan G, Lyu Q, Li F, Zhang W
Oncogene 2025 Jun;44(22):1731-1745

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Treadmill Exercise Modulates the Leptin/LepR/GSK-3β Signalling Pathway to Improve Leptin Sensitivity and Alleviate Neuroinflammation in High-Fat Diet-Fed APP/PS1 Mice.

Wang J, Liao M, Tong Z, Yuan S, Hu Z, Chen Z, Zeng F, Zou R, Chen D, Chen G, Wang Z, Liu W
Molecular neurobiology 2025 Aug;62(8):9651-9669

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Targeting Bacteria-Induced Ferroptosis of Bone Marrow Mesenchymal Stem Cells to Promote the Repair of Infected Bone Defects.

Yuan K, Yang Y, Lin Y, Zhou F, Huang K, Yang S, Kong W, Li F, Kan T, Wang Y, Cheng C, Liang Y, Chang H, Huang J, Ao H, Yu Z, Li H, Liu Y, Tang T
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Oct;11(39):e2404453

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Leptin and insulin synergize with PIK3CA mutation to enhance PD-L1 mediated immunosuppression in thyroid cancer.

Wu K, Chen Y, Guo R, Zeng Q, Yu Y
Experimental cell research 2024 Oct 1;442(2):114229

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Granulocyte colony-stimulating factor mediates bone loss via the activation of IL-1β/JNK signaling pathway in murine Staphylococcus aureus-induced osteomyelitis.

Song M, Deng M, Peng Z, Dai F, Wang Y, Shu W, Zhou X, Zhang J, Hou Y, Yu B
International immunopharmacology 2024 Nov 15;141:112959

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LRP1-deficient leptin receptor-positive cells in periodontal ligament tissue reduce alveolar bone mass by inhibiting bone formation.

Nishimura S, Kariya H, Gakiya Y, Shinohara R, Nakamura Y, Mizoguchi T, Ohashi A, Motoyoshi M, Ninomiya T
Archives of oral biology 2024 Feb;158:105853

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Regulatory role of Heparan sulfate in leptin signaling.

Nagai N, Shioiri T, Hatano S, Sugiura N, Watanabe H
Cellular signalling 2024 Dec;124:111456

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Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer.

Venkata PP, Jayamohan S, He Y, Alejo S, Johnson JD, Palacios BE, Pratap UP, Chen Y, Liu Z, Zou Y, Lai Z, Suzuki T, Viswanadhapalli S, Weintraub ST, Palakurthi S, Valente PT, Tekmal RR, Kost ER, Vadlamudi RK, Sareddy GR
Cancer letters 2023 Oct 28;575:216383

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Splicing factor YBX1 regulates bone marrow stromal cell fate during aging.

Xiao Y, Cai GP, Feng X, Li YJ, Guo WH, Guo Q, Huang Y, Su T, Li CJ, Luo XH, Zheng YJ, Yang M
The EMBO journal 2023 May 2;42(9):e111762

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In Vivo Evaluation of Bone Regenerative Capacity of the Novel Nanobiomaterial: β-Tricalcium Phosphate Polylactic Acid-co-Glycolide (β-TCP/PLLA/PGA) for Use in Maxillofacial Bone Defects.

Ramanathan M, Shijirbold A, Okui T, Tatsumi H, Kotani T, Shimamura Y, Morioka R, Ayasaka K, Kanno T
Nanomaterials (Basel, Switzerland) 2023 Dec 28;14(1)

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Celastrol inhibits the proliferation and migration of MCF-7 cells through the leptin-triggered PI3K/AKT pathway.

Chen P, Wang B, Li M, Cui C, Liu F, Gao Y
Computational and structural biotechnology journal 2022;20:3173-3181

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Presence of leptin and its receptor in the ram reproductive system and in vitro effect of leptin on sperm quality.

Gao Y, Zhao G, Song Y, Haire A, Yang A, Zhao X, Wusiman A
PeerJ 2022;10:e13982

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Epiregulin as an Alternative Ligand for Leptin Receptor Alleviates Glucose Intolerance without Change in Obesity.

Song NJ, Lee A, Yasmeen R, Shen Q, Yang K, Kumar SB, Muhanna D, Arnipalli S, Noria SF, Needleman BJ, Hazey JW, Mikami DJ, Ortega-Anaya J, Jiménez-Flores R, Prokop J, Ziouzenkova O
Cells 2022 Jan 26;11(3)

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Effects of carotid baroreceptor stimulation on aortic remodeling in obese rats.

Yu Q, Shu L, Wang L, Gao K, Wang J, Dai M, Cao Q, Zhang Y, Luo Q, Hu B, Dai D, Chen J, Bao M
Nutrition, metabolism, and cardiovascular diseases : NMCD 2021 May 6;31(5):1635-1644

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919 syrup inhibits ROS-mediated leptin-induced anorexia by activating PPARγ and improves gut flora abnormalities.

Xing JW, Chen MM, Tian XY, Pan DQ, Peng XH, Gao PF
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2021 Jun;138:111455

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PP2A in LepR+ mesenchymal stem cells contributes to embryonic and postnatal endochondral ossification through Runx2 dephosphorylation.

Yen YT, Chien M, Wu PY, Hung SC
Communications biology 2021 Jun 2;4(1):658

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Immunotherapy against angiotensin II receptor ameliorated insulin resistance in a leptin receptor-dependent manner.

Zheng J, Ding J, Liao M, Qiu Z, Yuan Q, Mai W, Dai Y, Zhang H, Wu H, Wang Y, Liao Y, Chen X, Cheng X
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Jan;35(1):e21157

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Leptin Receptor (LEPR) promotes proliferation, migration, and invasion and inhibits apoptosis in hepatocellular carcinoma by regulating ANXA7.

Huang H, Zhang J, Ling F, Huang Y, Yang M, Zhang Y, Wei Y, Zhang Q, Wang H, Song L, Wu Y, Yang J, Tang J
Cancer cell international 2021 Jan 4;21(1):4

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Feasibility of Application of the Newly Developed Nano-Biomaterial, β-TCP/PDLLA, in Maxillofacial Reconstructive Surgery: A Pilot Rat Study.

Toda E, Bai Y, Sha J, Dong QN, Ngo HX, Suyama T, Miyamoto K, Matsuzaki Y, Kanno T
Nanomaterials (Basel, Switzerland) 2021 Jan 25;11(2)

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Leptin, Leptin Receptor, KHDRBS1 (KH RNA Binding Domain Containing, Signal Transduction Associated 1), and Adiponectin in Bone Metastasis from Breast Carcinoma: An Immunohistochemical Study.

Maroni P, Luzzati A, Perrucchini G, Cannavò L, Bendinelli P
Biomedicines 2020 Nov 17;8(11)

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Acid sphingomyelinase downregulation alleviates vascular endothelial leptin resistance in rats.

Cai BB, Lu YN, Xu M
Acta pharmacologica Sinica 2020 May;41(5):650-660

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Knockdown of Leptin Receptor Affects Macrophage Phenotype in the Tumor Microenvironment Inhibiting Breast Cancer Growth and Progression.

Gelsomino L, Naimo GD, Malivindi R, Augimeri G, Panza S, Giordano C, Barone I, Bonofiglio D, Mauro L, Catalano S, Andò S
Cancers 2020 Jul 27;12(8)

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JAK2/STAT3 regulates estrogen-related senescence of bone marrow stem cells.

Wu W, Fu J, Gu Y, Wei Y, Ma P, Wu J
The Journal of endocrinology 2020 Apr;245(1):141-153

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Advantage of Alveolar Ridge Augmentation with Bioactive/Bioresorbable Screws Made of Composites of Unsintered Hydroxyapatite and Poly-L-lactide.

Sukegawa S, Kawai H, Nakano K, Takabatake K, Kanno T, Nagatsuka H, Furuki Y
Materials (Basel, Switzerland) 2019 Nov 8;12(22)

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Leptin mediates the effects of melatonin on female reproduction in mammals.

Lv D, Tan T, Zhu T, Wang J, Zhang S, Zhang L, Hu X, Liu G, Xing Y
Journal of pineal research 2019 Apr;66(3):e12559

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Anti-Obesity Effect of Chitosan Oligosaccharide Capsules (COSCs) in Obese Rats by Ameliorating Leptin Resistance and Adipogenesis.

Pan H, Fu C, Huang L, Jiang Y, Deng X, Guo J, Su Z
Marine drugs 2018 Jun 5;16(6)

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Ablation of β,β-carotene-9',10'-oxygenase 2 remodels the hypothalamic metabolome leading to metabolic disorders in mice.

Guo X, Wu L, Lyu Y, Chowanadisai W, Clarke SL, Lucas EA, Smith BJ, He H, Wang W, Medeiros DM, Lin D
The Journal of nutritional biochemistry 2017 Aug;46:74-82

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Leptin signaling enhances cell invasion and promotes the metastasis of human pancreatic cancer via increasing MMP-13 production.

Fan Y, Gan Y, Shen Y, Cai X, Song Y, Zhao F, Yao M, Gu J, Tu H
Oncotarget 2015 Jun 30;6(18):16120-34

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

PC-3 cells were subjected to SDS PAGE followed by western blot with 20966-1-AP(LEPR antibody) at dilution of 1:300

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The LEPR antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human LEPR. This antibody recognizes human antigen. The LEPR antibody has been validated for the following applications: ELISA, WB, IHC, IF analysis.

Appendix

20966-1-AP.pdf

Show more

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate hyperplasia using 20966-1-AP(LEPR antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human prostate hyperplasia using 20966-1-AP(LEPR antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue