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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- MAB867-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Leptin R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human Leptin R in direct ELISAs and Western blots. In Western blots, cross reactivity with recombinant mouse Leptin R is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P48357- Isotype
- IgG
- Antibody clone number
- 52263
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Leptin enhances cytokine/chemokine production by normal lung fibroblasts by binding to leptin receptor.
Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis.
Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.
Watanabe K, Suzukawa M, Arakawa S, Kobayashi K, Igarashi S, Tashimo H, Nagai H, Tohma S, Nagase T, Ohta K
Allergology international : official journal of the Japanese Society of Allergology 2019 Sep;68S:S3-S8
Allergology international : official journal of the Japanese Society of Allergology 2019 Sep;68S:S3-S8
Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis.
Liang G, Gao W, Liang A, Ye W, Peng Y, Zhang L, Sharma S, Su P, Huang D
PloS one 2012;7(5):e36648
PloS one 2012;7(5):e36648
Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages.
Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E474-82
American journal of physiology. Endocrinology and metabolism 2009 Aug;297(2):E474-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human Leptin R by Western Blot. Western blot shows 25 ng of Recombinant Human Leptin R Fc Chimera (Catalog # 389-LR) and Recombinant Mouse Leptin R Fc Chimera (Catalog # 497-LR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Leptin R Monoclonal Antibody (Catalog # MAB867) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Leptin R at approximately 155-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Leptin R in Human Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Human Leptin R Monoclonal Antibody (Catalog # MAB867, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B).
