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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Immunohistochemistry [1]
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- Product number
- A00350 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Leptin Receptor/LEPR Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Leptin R/LEPR detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. Leptin R/LEPR information: Molecular Weight: 132494 MW; Subcellular Localization: Cell membrane; Single-pass type I membrane protein; Tissue Specificity: Isoform A is expressed in fetal liver and in hematopoietic tissues and choroid plexus. In adults highest expression in heart, liver, small intestine, prostate and ovary. Low level in lung and kidney. Isoform B is highly expressed in hypothalamus.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Leptin decreases the transcription of BK(Ca) channels and Gs to Gi protein-ratio in late pregnant rat uterus.
Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NFκB Signaling in the Hypothalamus of Aging Mice.
Pavithra S, Kishor Kumar DG, Ramesh G, Panigrahi M, Sahoo M, Madhu CL, Singh TU, Kumar D, Parida S
Gene 2024 Jan 15;891:147831
Gene 2024 Jan 15;891:147831
Long-Term l-Serine Administration Reduces Food Intake and Improves Oxidative Stress and Sirt1/NFκB Signaling in the Hypothalamus of Aging Mice.
Zhou X, Zhang H, He L, Wu X, Yin Y
Frontiers in endocrinology 2018;9:476
Frontiers in endocrinology 2018;9:476
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Leptin Receptor/LEPR using anti-Leptin Receptor/LEPR antibody (A00350). Leptin Receptor/LEPR was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Leptin Receptor/LEPR Antibody (A00350) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
- Additional image
