LS-B2745

antibody from LSBio

Targeting: AKR1A1 ALR, DD3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B2745

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B2745, RRID:AB_2224145

Product name

IHC-plus™ AKR1A1 Antibody (aa313-325) LS-B2745

Antibody type

Polyclonal

Description

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Hamster, Horse, Porcine, Rabbit, Simian, Xenopus

Host

Goat

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Antibody staining (1 ug/ml) of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293 overexpressing AKR1A1 (RC200302) and probed with the antibody (mock transfection in first lane).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Aldehyde Reductase antibody (0.1µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.