TA500740

antibody from OriGene

Targeting: AKR1A1 ALR, DD3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500740

Provider

OriGene

Proper citation

OriGene Cat#TA500740, RRID:AB_11128818

Product name

Anti-AKR1A1 mouse monoclonal antibody, clone OTI9F1 (formerly 9F1)

Antibody type

Monoclonal

Description

Anti-AKR1A1 mouse monoclonal antibody, clone OTI9F1 (formerly 9F1)

Host

Mouse

Conjugate

Unconjugated

Epitope

AKR1A1

Isotype

IgG

Antibody clone number

OTI9F1

Vial size

100 µl

Concentration

0.7 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (RC200302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-AKR1A1 monoclonal antibody.(1:200)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-AKR1A1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500740, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-AKR1A1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500740, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric analysis of Jurkat cells, using anti-AKR1A1 antibody(TA500740),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).

Validation comment

FC