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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- TA500745 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500745, RRID:AB_11124431
- Product name
- Anti-AKR1A1 mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)
- Antibody type
- Monoclonal
- Description
- Anti-AKR1A1 mouse monoclonal antibody, clone OTI6E3 (formerly 6E3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- AKR1A1
- Isotype
- IgG
- Antibody clone number
- OTI6E3
- Vial size
- 100 µl
- Concentration
- 0.74 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKR1A1 (RC200302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKR1A1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-AKR1A1 mouse monoclonal antibody (TA500745) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKR1A1(RC200302).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-AKR1A1 mouse monoclonal antibody (TA500745).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-AKR1A1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500745, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either pCMV6-ENTRY AKR1A1(RC200302)(Red) or empty vector control plasmid(Blue) were immunostained with anti-AKR1A1 mouse monoclonal(TA500745), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells, using anti-AKR1A1 antibody(TA500745),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-AKR1A1 antibody(TA500745),(Red) compared to a nonspecific negative control antibody(TA50011)(Blue).
- Validation comment
- FC
