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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00010327-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010327-M01, RRID:AB_2224130
- Product name
- Mouse Monoclonal AKR1A1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AKR1A1 - aldo-keto reductase family 1, member A1 (aldehyde reductase)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Two allelic variants of aldo-keto reductase 1A1 exhibit reduced in vitro metabolism of daunorubicin.
Mapping of phosphorylation-dependent anti-tau monoclonal antibodies in immunoblots using human tau-constructs synthesized by native chemical ligation.
Aldo-keto reductase 1C2 fails to metabolize doxorubicin and daunorubicin in vitro.
Bains OS, Takahashi RH, Pfeifer TA, Grigliatti TA, Reid RE, Riggs KW
Drug metabolism and disposition: the biological fate of chemicals 2008 May;36(5):904-10
Drug metabolism and disposition: the biological fate of chemicals 2008 May;36(5):904-10
Mapping of phosphorylation-dependent anti-tau monoclonal antibodies in immunoblots using human tau-constructs synthesized by native chemical ligation.
Singer D, Herth N, Kuhlmann J, Holland-Nell K, Beck-Sickinger AG, Hoffmann R
Biochemical and biophysical research communications 2008 Mar 7;367(2):318-22
Biochemical and biophysical research communications 2008 Mar 7;367(2):318-22
Aldo-keto reductase 1C2 fails to metabolize doxorubicin and daunorubicin in vitro.
Takahashi RH, Bains OS, Pfeifer TA, Grigliatti TA, Reid RE, Riggs KW
Drug metabolism and disposition: the biological fate of chemicals 2008 Jun;36(6):991-4
Drug metabolism and disposition: the biological fate of chemicals 2008 Jun;36(6):991-4
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - Analysis of AKR1A1 expression in transfected 293T cell line by AKR1A1 monoclonal antibody (M01), clone 1A11-2A4.Lane 1: AKR1A1 transfected lysate(36.6 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - AKR1A1 monoclonal antibody (M01), clone 1A11-2A4 Analysis of AKR1A1 expression in HL-60.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - Detection limit for recombinant GST tagged AKR1A1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AKR1A1 Antibody (1A11-2A4) [H00010327-M01] - Analysis of monoclonal antibody to AKR1A1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.75 ug/ml.
